摘要
目的观察人脂肪基质干细胞(hADSC)复合藻酸钙凝胶体外构建工程软骨的可行性。方法取临床整形外科超声乳化的成人脂肪组织溶液,经消化、分离、培养得到hADSC,经成软骨诱导培养后与藻酸钠凝胶复合,使细胞终浓度为5×106/mL,然后滴入浓度200 mmol/L CaCl2溶液中,固化15 min形成藻酸钙凝珠,将其在离心管中悬浮培养8周,行大体、苏木精-伊红(HE)染色、Masson’s三色染色等组织学检测,观察组织工程软骨形成情况。结果 hADSC经成软骨诱导培养后复合藻酸钙凝胶可于体外较好地构建出组织工程软骨,形成软骨中有胶原及黏多糖等软骨特有基质成分。结论 hADSC可以作为软骨组织工程较理想的种子细胞,为软骨缺损的临床修复治疗提供了新的治疗方法。
Objective To study the feasibility of constructing tissue engineered cartilage using human adipose derived stem cells（hADSC） and calcium alginate in vitro.Methods Human adult adipose tissues were obtained by liposuction procedures under local anesthesia,and then digested to isolate the hADSC.After cultured in the cartilage induction medium,the final concentration was adjusted to 5 × 106/mL.The cells were then mixed with 200 mmol/L calcium alginate gels to create tissue engineered cartilage in a centrifuge tube.After 8 weeks of culture in vitro,hematoxylin-eosin（HE） and Masson trichrome staining were performed to observe the construction of tissue engineered cartilage.Results Tissue engineered cartilage was constructed in vitro using hADSC in combination with calcium alginate gels after cultured in cartilage induction medium.The tissue engineered cartilage contained collagen and mucopolysaccharide.Conclusion It is demonstrated that hADSC could be used as a seed cell in cartilage tissue engineering and might provide a new way of repairing cartilage defect for clinical rehabilitation.
出处
《生物医学工程与临床》
CAS
2011年第5期408-412,F0003,共6页
Biomedical Engineering and Clinical Medicine
基金
国家自然科学基金资助项目(30772261)
关键词
人脂肪基质干细胞
软骨
组织工程
藻酸盐
human adipose derived stem cells
cartilage
tissue engineering
alginate
