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再生水中弗氏柠檬酸杆菌生物膜特性研究 被引量:5

STUDY ON THE CHARACTERISTIC OF CITROBACTER FREUNDII BIOFILM IN RECLAIMED WATER
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摘要 从使用再生水的某电厂冷却塔粘泥中分离出一株具有硫酸盐还原功能的弗氏柠檬酸杆菌.利用扫描电子显微镜(SEM)和能谱仪(EDS)对再生水中HSn70-1A和BFe30-1-1两种合金表面弗氏柠檬酸杆菌生物膜结构和成分进行表征,结果表明HSn70-1A成膜特性好于BFe30-1-1,两种合金表面生物膜中C、O等无机元素含量较大、基体金属元素含量下降较为明显.对不同时刻合金表面生物膜胞外聚合物(EPS)组分进行分析,多糖与蛋白质含量之比揭示出,在第3天和第7天两个关键期EPS的疏水性能对微生物膜的致密性和完整性产生重要影响,实验结果表明在两个关键期内HSn70-1A显示较好的疏水性能,使其比BFe30-1-1表现出较强的耐微生物腐蚀性能. A strain of Citrobacter freundii with sulfate-reducing function was isolated from the sludge at the bottom of cooling tower in a power plant using reclaimed water.Scanning electron microscopy(SEM) and energy dispersive spectrometry(EDS) were employed to investigate the characteristics of the biofilm formed on the surface of HSn70-1A and BFe30-1-1 in reclaimed water.The results show that the characteristics of biofilm-forming for HSn70-1A was better than BFe30-1-1.In biofilm the contents of C,O and other inorganic elements were relatively high,while the contents of matrix elements significantly decreased.The components of extracellular polymer substances(EPS)in the bio-film formed on the surface of copper alloy at the different time were analyzed.Differences in the ratio of polysaccharide to protein revealed that the 3rd day and 7th day were two critical periods for the formation of biofilm.During these two periods,EPS from HSn70-1A showed better hydrophobic properties than EPS from BFe30-1-1,which made HSn70-1A more resistant to microorganism corrosion.
出处 《环境化学》 CAS CSCD 北大核心 2011年第10期1687-1694,共8页 Environmental Chemistry
基金 河北大唐国际张家口热电有限责任公司基金(CX10001)资助
关键词 弗氏柠檬酸杆菌 微生物腐蚀 生物膜特性 胞外聚合物 硫酸盐还原菌 Citrobacter freundii microbiologically induced corrosion biofilm characteristics extracellular polymer substance(EPS) sulfate-reducing bacteria(SRB).
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