摘要
目的:克隆黄芩延长因子1a全长cDNA序列,并分析温度对其表达水平的影响。方法:通过构建黄芩全长cDNA文库克隆黄芩延长因子1a,利用生物信息学手段分析该基因的序列特征;利用RT-PCR分析温度对黄芩延长因子1a基因表达水平的影响。结果:黄芩延长因子1a基因长1 672 bp,开放阅读框位于97～1 446,共449个氨基酸,具有典型的延长因子1a结构域;高温和低温处理后,黄芩EF1a的转录水平均显著提高。结论:黄芩EF1a的克隆为进一步研究其在黄芩生长代谢过程中的功能和作用提供了基础。
Objective： To clone elongation factor-1a（EF-1a） gene cDNA and analyse its expression level of the temperature effect.Method： Elongation factor-1a（EF-1a） gene was cloned through constructing cDNA library of Scutellaria baicalensis,and its characteristics was analysed by bioinformatics method.Its expression level of the temperature effect was analysed by using RT-PCR.Result：The length of elongation factor-1a（EF-1a） gene of Scutellaria baicalensis was 1 672 bp,the open reading box located in 97-1 446 with 449 aminos.It was a typical elongation factor domain.The transcription levels of SbEF1a were significantly improved with high and low temperature processing.Conclusion： 1a（EF-1a） gene of S.baicalensis was a elongation factor,participating in translation of protein.Its expression controled by environmental temperature,and its transcription level is significantly increased after high and low temperature processing.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第20期126-128,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国博士后基金特别资助项目(201003228)
北京市科技新星项目(2008B62)
关键词
黄芩
延长因子
表达分析
Scutellaria baicalensis
elongation factor
gene expression
