摘要
对分离自浙江各地的草莓炭疽病菌进行形态特征、培养性状等牛物学特性研究并测定ITS序列,结果表明:浙江各地引起草莓炭疽病的优势菌株为Colletotrichumgloeosporioides,该病原菌最适在PDA和PSA培养基上生长,菌丝生长和孢子萌发的最适温度为25~30℃,培养基的最适pH值为6.0~7.0,但孢子在pH值6.0时萌发率最高。以蔗糖和果糖为碳源,硝酸钾为氮源时生长速率最快。
Morphologic traits, cultural characteristics and rDNA ITS sequences were studied for the pathogens of strawberry anthracnoses collected in Zhejiang Province. The results showed that Colletotrichum gloeosporioides was the predominant pathogen causing the disease in Zhejiang. PDA and PSA culture media were suitable for the growth of C. gloeosporioides and the optimal temperature was 25 -30℃. It was found that spore germination rate was the highest in pH 6. 0, however, the suitable pH to maintain mycelium growth was 6.0 -7.0. The growth rate of mycelium was optimized using sucrose and fructose as carbon source and potassium nitrate as nitrogen source.
出处
《浙江农业学报》
CSCD
北大核心
2011年第5期937-941,共5页
Acta Agriculturae Zhejiangensis
基金
浙江省科技厅重点项目(2009C12073)
关键词
草莓
炭疽病菌
病原鉴定
生物学
strawberry
anthracnose
pathogen identification
biology