摘要
目的探讨脐带间充质干细胞能否在体内外分化为内皮细胞，参与血管新生。方法体外实验采用内皮细胞生长因子和碱性成纤维细胞生长因子对脐带间充质干细胞进行诱导，观察其形态变化。体内实验将脐带间充质干细胞移植到小鼠后肢缺血模型中，采用免疫组织化学鉴定细胞在体内的迁移和分化，激光多普勒血流成像仪鉴定缺血局部血流恢复情况。结果在体外脐带间充质干细胞能够形成血管网样结构。在体内脐带间充质干细胞能够分化为内皮细胞，表达CD31抗体，参与实验组的动物血管重建。与小鼠的血管网络发生整合，实验组的动物后肢血流灌注明显好于对照组。结论脐带间充质干细胞能够分化为内皮细胞，参与血管新生，为治疗性血管新生提供新的细胞选择。
Objective Myocardial infarction and subsequent heart failure remain the most dominant health challenges worldwide. Therapeutic angiogenesis has emerged as a potential novel treatment for severe ischemic heart disease and there is increasing evidence that cell transplantation may improve the perfusion and contractility of myocardium in animal models. This study was designed to examine the endothelial growth potential and whether transplantation of human umbilical cord derived mesenchymal stem cells can improve local blood flow in a mouse ischemic hindlimb model. Methods The mesenchymal stem ceils derived from human umbilical cord of passage 5 were differentiated in an endothelial differentiation medium containing vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） in vitro. Samples were observed for 2 weeks. The human umbilical cord derived mesenchymal stem cells were transplanted into a hindlimb ischemia mouse model in vivo. Four weeks later, immunofluence was used to identify the migration and differentiation of the transplanted cells towards endothelial linage. Laser Doppler perfusion image was used to evaluate the local blood flow of the hindlimb. Results Results After incubation with VEGF and bFGF, the human umbilical cord derived mesenchymal stem ceils started to form interconnec- ted clusters and a network was formed. Four weeks after transplantation, the transplanted ceils were sprouting from the local injection and differentiated into endothelial cells, contributed to the recovery of local blood flow obviously as compared with control group. Conclusion Human umbilical cord derived mesenchymal stem cells have the ability to differentiate into endothelial cells, contribute to the local angiogenesis in a hindlimb ischemia mouse model and represent a new source for therapeutic angiogenesis for clinical applications.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
2011年第9期553-555,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
基金
本课题受江苏省自然科学基金资助（BK2008076）.
关键词
内皮
血管
细胞分化
干细胞
新生血管化
生理性
Endothelium, vascular Cell differentiation Stem cells Neovascularization,physiologic
