摘要
目的:氢化可的松诱导的大鼠阳虚模型是目前较为公认的阳虚证模型,然而对氢化可的松剂量的选择尚未有统一结论。由于氢化可的松剂量可能是影响造模成功与否的重要因素,为比较不同造模剂量下模型状态的异同,在分析本实验室前期建立的多批不同剂量氢化可的松诱导大鼠阳虚状态模型数据的基础上,本文引入Ridit分析法,对大量实验数据进行挖掘,分析剂量对于模型的影响。方法:收录2.5、3.75、10和20mg/kg4个剂量氢化可的松造模的共27批实验数据,标准化后进行数据资料类型转化,继而运用Ridit分析法,计算并比较神经-内分泌系统、免疫系统、肝肾功能和物质能量代谢系统等方面的共19个指标的变化趋势。结果:从模型整体状态考察,2.5、3.75、10、20mg/kg4个剂量氢化可的松建立的阳虚模型之间差异不明显,提示造模剂量的改变不会对该模型状态造成显著改变。从指标变化趋势角度看,不同指标在不同剂量下表现出一定差异,例如性腺轴激素雌二醇和睾酮在2.5mg/kg剂量下与正常组比较差异较大,更能体现阳虚状态的特点;免疫系统指标免疫球蛋白G、免疫球蛋白M和物质能量代谢系统指标三酰甘油、总胆固醇则分别在3.75和10mg/kg剂量下有更明显的变化,提示需根据预期研究的生理生化指标确定与之相匹配的造模剂量。结论:Ridit分析有利于不同剂量下氢化可的松阳虚模型状态的综合分析,该法也为阳虚模型状态的深入研究奠定了基础。
Objective： Hydrocortisone-induced yang-deficiency animal model has now become the generally accepted model of yang deficiency. However, assessing the most appropriate dose of hydrocortisone is a long-term challenge. For analyzing the modeling dose, the authors have built several kinds of yang-deficiency models induced by hydrocortisone at different doses, and analyzed the experimental data with various mathematical statistical methods. In order to discuss the effects of the modeling dose on the basis of previous research, the authors introduced Ridit analysis.Methods： After categorizing 27 batches of experiments with four different doses （including 2.5, 3.75, 10 and 20 mg/kg）, the data were standardized and the data type was changed to meet the needs of subsequent calculations. Then, by using Ridit analysis, the authors compared the variation of 19 biochemistry indexes involving nervous-endocrine system, immune system, metabolic system and the function of the liver and kidney, so as to analyze the similarities and differences of those yang-deficiency models mentioned above. The effects of modeling dose were then discussed.Results： With regard to the overall state of the animal model, the difference between the models induced by different doses of hydrocortisone （2.5, 3.75, 10 and 20 mg/kg） showed no statistical significance, indicating that differences in hydrocortisone dose barely lead to significant changes in yang-deficiency models. For the trends of changing in different indexes, indexes had different performances when the doses differs： gonad indexes （estradiol and testosterone） showed better performance in 2.5 mg/kg group, immunological indexes （immunoglobulin M and immunoglobulin G） showed better performance in 3.75 mg/kg group and metabolic indexes （triglyceride and total cholesterol） showed better performance in 10 mg/kg group, etc. This indicates that the dose of hydrocortisone should be determined according to the research purposes.Conclusion： Ridit analysis can be used as
出处
《中西医结合学报》
CAS
2011年第9期941-947,共7页
Journal of Chinese Integrative Medicine
基金
国家重点基础研究发展计划(973计划)资助项目(No.2007CB512605)
关键词
RIDIT分析
阳虚
氢化可的松
疾病模型
动物
数据说明
统计
大鼠
Ridit analysis
yang deficiency
hydrocortisone
disease models
animal
data interpretation
statistical
rats
