摘要
我国流行的猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)包括美洲型经典毒株和变异株。本研究应用RT-PCR、直接免疫荧光(direct immunofluorescence asay,DFA)及病毒分离3种检测方法对99份不同类型的猪繁殖与呼吸综合征临床疑似病料和人工感染病料进行检测。结果显示,RT-PCR检测试剂盒敏感性最高,DFA鉴别诊断试剂盒次之,病毒分离最差。DFA与病毒分离、RT-PCR的总符合率分别为92%和85.5%。RT-PCR灵敏性高,可作为PRRSV鉴别诊断的首选方法;DFA鉴别诊断法所需时间较短、特异性好,但需要一定的操作经验;病毒分离敏感性低、操作繁琐,阳性分离需要其它方法验证,不适合于PRRSV的鉴别诊断。
Three diagnostic method including the virus isolation and identification （VI）, direct immunofluorescence assay （DFA） and reverse transcriptase polymerase chain reaction （RT-PCR） were employed to detect 99 specimens , parallelly. The results showed that the coincidence between DFA and VI, DFA and RT-PCR were 92.5% and 92.2%, respectively. RT-PCR was the highest sensitivity and specificity, which could be used as the first choice for laboratory differential diagnosis. DFA was high specificity and fast, especially useful for PRRS＇s clinic detecting. The virus isolation＇s procedure was complicated, consumed long time, had a low sensitivity and positive separation needed other methods to identification. Therefore virus isolation was not a suitable test for distinguish detection.
出处
《中国动物传染病学报》
CAS
2011年第4期71-75,共5页
Chinese Journal of Animal Infectious Diseases
基金
福建省科技计划项目(2008N0027)
福建省农科院创新团队项目(STIF-Y02)
