摘要
为提高核酸测量的准确性,种类繁多的核酸标准物质被研制出来。标准物质是保证量值准确性与可溯源性的“计量器具”,具有复现、保存和传递量值的功能,可以为核酸定性与定量检测过程的质量控制提供参考。准确可靠的定值方法是标准物质研制的重要基础,详细介绍了几种核酸标准物质测量方法,重点分析了不同测量方法的原理与应用特点,讨论了测量过程中可能存在的影响因素,为核酸标准物质的深入研究提供参考。
A great variety of nucleic acid reference materials have been developed to improve the accuracy of nucleic acid measurement.A reference material is a“measuring instrument”that can ensure the accuracy and traceability of measurement,with the functions of reproducing,preserving,and disseminating measurement values.Reference materials can provide a frame of reference for quality control of qualitative and quantitative detection processes for nucleic acid.Accurate and reliable certification methods are an important basis for the development of reference materials.This article provides details on several methods of measuring nucleic acid reference materials,with a focus on the principles and application characteristics of the methods and a discussion on influencing factors that may affect the measurement.
作者
陈桂芳
欧阳艳艳
杨佳怡
高运华
CHEN Guifang;OUYANG Yanyan;YANG Jiayi;GAO Yunhua(Shenyang Chemical Industry University,Shenyang 110142,China;National Institute of Metrology,Beijing 100029,China)
出处
《计量科学与技术》
2021年第6期25-33,共9页
Metrology Science and Technology
基金
中国计量科学研究院基本科研业务费项目(AKYZZ2023)。
