摘要
目的探讨N-乙酰半胱氨酸（N—acetylcysteine，NAC）对大鼠胆道梗阻所致肝功能损伤的保护作用及其机制。方法Wistar大鼠72只随机均分成3组：（1）胆道结扎＋NAC组（DBL＋NAC，n=24）：开腹结扎并切断胆总管，建模成功后经腹腔注射NAC（150mg·kg-1·d-1）连续注射7d；（2）胆道结扎组（DBL组，n=24）；（3）假手术组（SO组，n=24）：仅行开腹游离胆总管不予结扎和切断。建模成功后1、3、5、7d每组分别活杀6只大鼠，取静脉血及肝组织，检测肝功能、血浆肿瘤坏死因子a（TNF—a）在各时相点的变化并采用Griess法检测一氧化氮（NO）产生情况。结果在DBL组、DBL＋NAC组谷-草转氨酶（AST）、血清谷丙转氨酶（ALT）、总胆红素（TBIL）、直接胆红素（DBIL）均随胆道梗阻时间延长而升高，但DBL组AST、ALT在各时间点均较DBL＋NAC组明显升高（P〈0．05），而TBIL、DBIL在这两组间无明显差异（P〉0．05）。DBL组和DBL＋NAC组TNF-a、NO浓度变化也随梗阻时间延长而升高，但DBL组较DBL＋NAC组TNF—a、NO浓度升高更明显（P〈0．05）。结论N-乙酰半胱氨酸能有效改善胆道梗阻所致肝损害，并有可能是通过下调肝组织中TNF-a、NO的表达这一途径实现的。
Objective To investigate the mech-anisms of N-acetylcysteine protection against liver injury caused by biliary tract obstruction in rats. Methods Seventy-two Wistar rats were divided randomly into the following three groups： （1） Rats received choledochus ligationon and N-acetylcysteine by intraperitoneal injection （DBL＋ NAC） ; （2） Rats received choledochus ligationon only; （3） sham operation. Six rats were killed each time after 1 d, 3 d, 5 d and7 d and their liver function was tested by TBA-40FR Automatic Chemistry Analyzer. In addition, NO production was detected by Griess Reagent System. Results When the period of biliary duct obstruction is extended, serum AST, ALT, TBIL, DBIL, NO and TNF-a increase but the increase in serum ALT, AST, NO and TNF-a （P〈0.05） is more prominent in group DBL than group DBL＋NAC at any given time. Conclusions N-acetylcysteine can effectively protect against liver injury in rats with obstructive jaundice, and this may be achieved through down-regulation of TNF-a and NO in hepatocytes.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2011年第9期768-770,共3页
Chinese Journal of Hepatobiliary Surgery
