摘要
目的:探讨乙酰半胱氨酸对大鼠酒精性肝损伤的保护作用。方法:SD大鼠70只,随机分成6组:正常组(n=10,等渗盐水灌胃和腹腔注射)、模型组(n=12,酒精8.8g·kg-1·d-1灌胃)、治疗组(n=12,酒精8.8g·kg-1·d-1灌胃,造模同时腹腔注射0.156、0.312、0.625g/kg乙酰半胱氨酸)、阳性对照组(n=12,酒精8.8g·kg-1·d-1灌胃,造模同时腹腔注射硫普罗宁16.5mg·kg-1·d-1)。10周末处死大鼠,检测血清ALT、AST活性和肝组织谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量,观察肝组织病理变化。结果:模型组大鼠血清ALT、AST活性、肝组织MDA水平较正常组升高,肝组织GSH-PX、SOD较正常组降低,差异有统计学意义(P<0.05);治疗组、阳性对照组与模型组相比,ALT、AST、MDA有明显的降低,肝组织GSH-PX、SOD水平有明显的升高,差异有统计学意义(P<0.05)。HE染色显示模型组大鼠肝细胞浆出现不同程度脂肪变性,肝小叶可见肝细胞点状坏死,治疗组和阳性对照组脂肪变性和炎症程度轻于模型组。结论:乙酰半胱氨酸对大鼠酒精性肝损伤具有一定的保护作用。
AIM： To reproduce an experimental model of alcoholic in rats and to investigate the protective effects of N-acetylcysteine on alcoholic liver disease. METHODS： 70 male Sprague-Dawley rats were randomly divided into 6 groups： control group （gastric infusion and intraperitoneal injection with normal saline）, alcohol group （gastric infused with 55%of ethanol once a day with a dose of 8.8 g/kg for 10 weeks）, NAC groups （gastric infusion with alcohol same as in the alcohol group and intraperitoneal injection with NAC with different doses）, positive control group （gastric infusion with alcohol same as in the alcohol goup and intraperitoneal injection with tiopronin of 16.5 mg/kg）. At the end of 6 weeks blood samples were collected through caudal vein. At the end of 10 weeks the rats were sacrificed then blood samples were col- lected. Liver samples were obtained for detection and for routine histological examination. RESULTS： （ 1 ）The levels of the two transaminases（ALT＆AST）, and Malondialdehyde were elevated in alcohol group, and the Glutathione peroxidase and superoxide dismutase levels were lower than control group. The difference is significant. NAC significantly mitigated the increase of ALT, MDA, AST, and significantly elevated GSH-PX, SOD induced by the alcohol. （2）Histological changes of the liver injury that piecemeal or focal necrosis of hepatocytes was present in the centrilobular area. In the NAC and positive control group, the severity of the histological changes was significantly milder. CONCLUSION： The results of this study revealed that N-acetylcysteine protected from alcoholic liver disease.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2008年第5期517-520,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
杭州市卫生局重点资助项目(2006A12)
