摘要
从胎龄3个月左右的山羊胚胎中分离出背根神经节,采用组织块培养法,利用血清培养和无血清培养,并在非神经细胞抑制剂作用的条件下,观察了山羊背根神经节神经细胞在体外的生长状况,采用免疫荧光和RT-PCR技术对神经细胞表面标志神经元特异性磷酸化酶(NSE)进行了鉴定。结果显示,接种48h后的细胞从组织中迁出,并长出突起,经阿糖胞苷作用后,神经细胞与非神经细胞分层;经免疫荧光鉴定,上层细胞为神经细胞。随着时间的增加,神经细胞突起延长并交错成网状,至第6～10天神经细胞形态最为成熟饱满,随后逐渐出现细胞老化,神经细胞最长可生存32d。表明本试验成功分离培养得到了山羊背根神经节神经细胞。
The aim of the present study was to get the goat dorsal root ganglia（DRG） cells from the goat embryo.The DRG was isolated from goat embryo of three months old,and then were cultured with tissue culture method using both serum and serum-free medium.The growth of the DRG cells was observed in vitro under the effect of non-neuro cell inhibitor.NSE,the neuro cell surface markers was detected with immunofluorescence and RT-PCR.The cells were immigrated from the tissue on hour 48 after incubation,and the neurite was visible.After the effect of cytarabine,neurons and non-neuro cell were demixing,and neurons was identified in the over layer by immunofluorescence.The neurites were distinguished extended and connected into nets as time went on.Cells were fully developed on day 6 to 10,and then began aging;and it was observed that 32 days were the longest life span.The DRG cells were successfully isolated from the goat embryo and cultured in vitro.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2011年第9期941-944,共4页
Chinese Veterinary Science
基金
陕西省农业推广专项(14220201)
关键词
神经元
体外培养
背根神经节
山羊
neuron
culture in vitro
dorsal root ganglia
goat
