摘要
用8天鸡胚大脑半球进行分散神经细胞培养,培养6天时神经细胞占83%。此时将培养液换为不含葡萄糖的Earle氏缓冲盐水,置95%氮与5%CO_2中1—4小时,可以观察到神经细胞肿胀,失去折光性,台盼蓝着染比例增加,乳酸脱氢酶(LDH)漏出加快,γ-氨基丁酸(GABA)高亲和摄取能力下降。其中GABA摄取较早表现出严重变化。若孵育液中含糖缺氧,或有氧无糖,均不产生明显的变化。上述结果类似整体动物脑缺血后的神经细胞变化,故这一体外模型可用来研究缺血性中枢神经细胞损伤,损伤后恢复及药物的保护作用。
The dissociated monotypic nerve cell culture was established using 8 day chick embryonic cerebral hemispheres. On the 6th day in vitro, the culture medium was substituted by Earle's ballanced salt solution without glucose, then the cells were incubated in an atmosphere of 95% nitrogen and 5% CO_2. After 1 to 4 hours, increasing number of nerve cells became swollen, lost their character of optical refraction under phase contrast microscope, and were stained with trypan blue. Their leakage of LDH increased and their ability of high affinity uptake of GABA decreased. The reduction of GABA uptake took place earlier and more remarkable than that of other parameters. No significant change was observed if the ceils were incubated with glucose but without oxygen or with oxygen but without glucose. The above mentioned alterations were similar to nerve cell changes following cerebral ischemia in vivo. This model in vitro is useful in the study of ischemic nerve cell damage, their recovery and the protective effect of drugs.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1989年第5期293-297,共5页
Chinese Journal of Pathophysiology
关键词
脑缺血
神经元
培养基
体外
Cerebral ischemia
Neurons
In vitro
Culture media
