摘要
Background Effects of icariin on airway inflammation in asthmatic rats and the intervention of LPS induced inflammation are interfered with the machanism of icariin. Our study aimed to observe the effect of icariin on ovalbumin-induced imbalance of Th1/Th2 cytokine expression and its mechanism. Methods Sixty male SD rats were randomly divided into control group （PBS）, asthma group （ovalbumin （OVA）-induced） dexamethasone group, and OVA＋icariin low, medium and high dose groups （5, 10, 20 mg/kg, respectively）. Each group had ten rats. The model of OVA sensitization was a rat asthma model. Enzyme-linked immunosorbent assay （ELISA） method was used to observe the effects of icariin on interleukin-4 （IL-4） and inerferon y （IFN-y） in rats＇ lung tissue. Immunohistochemical staining was applied to detect the intervention effects of icadin on T cells （T-bet） and gatabinding protein 3 （GATA-3） in rat pulmonary tissue. Realtime RT-PCR was used to observe the intervention effects of icariin on T-bet and GATA-3 mRNA expression in rat pulmonary tissue and spleen lymphocytes. Western blotting was used to observe the icariin intervention effects on T-bet, GATA-3 and nuclear factor-Kappa B （NF-KB） p65 protein expressions in rat pulmonary tissue. Results The ELISA results from pulmonary tissue showed that IL-4 expression was significantly reduced （P 〈0.05）, while the IFN-y expression increased but not significantly when we compared OVA＋icariin medium and high dose groups with the asthma group. Immunohistochemical staining of pulmonary tissue showed that the GATA-3 decreased significantly while the T-bet staining did not change in the OVA＋icariin high dose group. In pulmonary tissue and spleen lymphocytes T-bet and GATA-3 mRNA expressions were significantly reduced （P 〈0.05） in icariin treatment groups compared with the asthma model group. GATA-3 and T-bet mRNA in rat spleen lymphocytes in the asthma group were higher than in the control group. GATA-3 mRNA expression in pulm
Background Effects of icariin on airway inflammation in asthmatic rats and the intervention of LPS induced inflammation are interfered with the machanism of icariin. Our study aimed to observe the effect of icariin on ovalbumin-induced imbalance of Th1/Th2 cytokine expression and its mechanism. Methods Sixty male SD rats were randomly divided into control group （PBS）, asthma group （ovalbumin （OVA）-induced） dexamethasone group, and OVA＋icariin low, medium and high dose groups （5, 10, 20 mg/kg, respectively）. Each group had ten rats. The model of OVA sensitization was a rat asthma model. Enzyme-linked immunosorbent assay （ELISA） method was used to observe the effects of icariin on interleukin-4 （IL-4） and inerferon y （IFN-y） in rats＇ lung tissue. Immunohistochemical staining was applied to detect the intervention effects of icadin on T cells （T-bet） and gatabinding protein 3 （GATA-3） in rat pulmonary tissue. Realtime RT-PCR was used to observe the intervention effects of icariin on T-bet and GATA-3 mRNA expression in rat pulmonary tissue and spleen lymphocytes. Western blotting was used to observe the icariin intervention effects on T-bet, GATA-3 and nuclear factor-Kappa B （NF-KB） p65 protein expressions in rat pulmonary tissue. Results The ELISA results from pulmonary tissue showed that IL-4 expression was significantly reduced （P 〈0.05）, while the IFN-y expression increased but not significantly when we compared OVA＋icariin medium and high dose groups with the asthma group. Immunohistochemical staining of pulmonary tissue showed that the GATA-3 decreased significantly while the T-bet staining did not change in the OVA＋icariin high dose group. In pulmonary tissue and spleen lymphocytes T-bet and GATA-3 mRNA expressions were significantly reduced （P 〈0.05） in icariin treatment groups compared with the asthma model group. GATA-3 and T-bet mRNA in rat spleen lymphocytes in the asthma group were higher than in the control group. GATA-3 mRNA expression in pulm
