摘要
目的通过对视网膜和视神经神经再生的标志物生长相关蛋白-43（GAP-43）的测定和闪光视觉诱发电位（F—VEP）的测定，观察810nm低能量镓铝砷（Ga-AlAs）激光对大鼠视神经钳夹伤后视神经再生的影响。方法健康成年Wistar大鼠88只，体重（180～220）g，分成激光治疗组40只、单纯损伤组32只、正常对照组16只，每组又分成1，3，6，9周4个时间点。标准的视神经钳夹伤模型制备成功后，激光治疗组行激光照射，照射参数为：光导子直径5mm，照射强度60mw，持续时间3min，经皮至视神经损伤处，每日照射1次。单纯损伤组及正常对照组在进行激光照射时，激光器无功率输出。激光治疗1，3，6，9周后测量视网膜、视神经GAP-43mRNA含量和GAP-43蛋白的表达。并且测量损伤前，损伤即刻，治疗1，3，6，9周后患侧眼F-VEP。结果大鼠视神经损伤后，F—VEP有很大变化，表现在N1、P1、N2波潜伏期延长，在视神经损伤即刻，潜伏期瞬间延长，随后有所恢复，第1～3周进行性延长，在第3～9周之间N1、P1、N2波潜伏期有所恢复。激光治疗组N1、P1、N2波的潜伏期也延长，但是明显小于单纯损伤组。正常的视网膜和视神经中几乎没有GAP-43蛋白的表达，视神经损伤后，GAP-43蛋白的表达在伤后第1周时达到高峰，随后下降，激光治疗后第1，3，6周与同一时间单纯损伤组GAP-43蛋白表达相比有所提高（P〈0．05），视网膜的GAP-43mRNA的含量与GAP-43蛋白表达相一致。结论810nm低能量镓铝砷激光能够促进大鼠视神经钳夹伤后视神经再生。
Objective To determine whether or not 810 nm low power Ga-A1-As laser treatment can stimu- late the regeneration of damaged optic nerves by measuring the expression of growth associated protein 43 （GAP-43） and flash-visual evoked potential （F-VEP）. Methods Eighty-eight Wistar rats weighing （180-220） g were ran- domly divided into a laser therapy group with 40 rats, an injury group with 32 rats, and a normal control group with 16 rats. Each group was subdivided into 1st, 3rd, 6th and 9th week subgroups. A standardized crushing of the optic nerve was applied to make the model. After this, the laser therapy group was treated for 3 minutes daily at 60 mW ap- plied transcutaneously to a 5 mm diameter spot on the injured optic nerve. The injury and normal control groups re- ceived the same treatment with no laser output. The expression of GAP-43 was detected by immunohistochemistry and RT-PCR after 1, 3, 6 and 9 weeks of treatment. F-VEP was measured pre-injury, immediately after injury and 1, 3, 6 and 9 weeks post injury. Results After the optic nerve was injured, obvious changes in F-VEP were detected, including significantly prolonged latencies of N1, P1 and N2 waves. The latency increased immediately after the optic nerve injured, and then recovered, but after 1 and 3 weeks the latency was still prolonged. There was significant re- covery from the 3rd to the 9th week. In the laser therapy group, the peak latencies of the N1, P1 and N2 waves were also prolonged, but the changes were less than those in the injury group. Expression of GAP-43 was hardly detectable in normal retinas and optic nerves. GAP-43 had its highest expression level at 1 week post-injury, and then de- creased. At the 1st, 3rd and 6th week post-injury, the expression of GAP-43 in the laser therapy group was signifi- cantly higher than in the injury group. GAP-43 mRNA content in the retina showed the same tendency as GAP-43 protein. Conclusion A 810 nm low power Ga-A1-As laser can promote neural repair and axonal regeneration after opti
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2011年第9期662-667,共6页
Chinese Journal of Physical Medicine and Rehabilitation
基金
辽宁省自然科学基金资助项目（20061007）
