摘要
利用超级杂交稻协优9308(协青早B×中恢9308)衍生的重组自交系(recombinant inbred line,RIL)群体及其分子连锁图谱,应用Windows QTL Cartographer2.5对施氮和不施氮条件下水稻株高(PH)和抽穗期(HD)进行了QTL分析。在2种氮水平下检测到9个株高QTL和8个抽穗期QTL,检测到4个影响2种环境下株高和抽穗期差值的QTL,单个QTL可解释的表型变异介于5.68%～18.40%之间;在第7染色体上RM5436附近和第8染色上RM5556～RM310区间检测到同时控制2种氮水平下株高和抽穗期的QTL,各位点的遗传效应贡献率较大,增效等位基因均来源于R9308,适用于分子标记辅助育种和聚合育种。在第2染色体上RM5916～RM166区间和第8染色体上RM2366～RM5767区间分别检测到1个影响2种氮水平下抽穗期差值和1个株高差值的QTL可能对水稻的氮素高效利用有直接贡献。
A recombinant inbred line（RIL）,derived from a super hybrid rice cross of XQZB/R9308 and its genetic linkage map were used to identify QTLs through Windows QTL Cartographer 2.5 for plant height（PH） and heading date（HD） under low and normal nitrogen conditions in rice.Nine QTLs for PH and eight QTLs for HD were detected under two nitrogen levels,four QTLs for trait differences of PH and HD were detected between the two environments,explaining 5.68-18.40% of phenotypic variation.The pleiotropic QTLs influencing PH and HD were detected near RM5436 on chromosome 7 and in the interval RM5556-RM310 on chromosome 8,which showed high contribution of genetic effects and the position alleles were all originated from R9308.These QTLs are favorable for marker-assisted selection.One QTL for HD difference and one QTL for PH difference were detected in the interval RM5916-RM166 on chromosome 2 and in the interval RM2366-RM5767 on chromosome 8,which were considered to directly contribute to nitrogen use efficiency in rice.
出处
《作物学报》
CAS
CSCD
北大核心
2011年第9期1525-1532,共8页
Acta Agronomica Sinica
基金
农业部作物种质资源保护项目(NB2011-2130135-27)
中央级公益性科研院所专项资金资助项目(2009RG001-3)
浙江省重点科技创新团队项目(2010R50024-16)资助
关键词
水稻
氮水平
株高
抽穗期
QTL分析
Rice
Nitrogen level
Plant height
Heading date
QTL analysis
