摘要
目的探讨碱性成纤维细胞因子（bFGF）与维生素C复合降解膜防止肌腱粘连的可行性。方法60只新西兰大耳白兔随机平均分成实验组和对照组。常规手术制备自体肌腱移植动物模型，实验组肌腱移植处包裹bFGF与维生素C复合降解膜，对照组不包裹。术后第3周常规HE染色和硝酸银染色分别观察2组成纤维细胞核和维生素c的变化，Luzex-F图像分析仪测定胶原纤维数量，术后第8周测定移植腱腱周粘连程度和最大抗拉力值。结果术后第3周，实验组成纤维细胞核和维生素C银染颗粒明显多于对照组；实验组肌腱内部胶原纤维数量（1230．5±56．4）根，明显多于对照组（998．4±26．2）根，差异有统计学意义（t=11．78，P〈0．001）。术后第8周，实验组腱周粘连程度（Ⅰ级为1根，Ⅱ级7根，Ⅲ级2根，Ⅳ级0根，Ⅴ级0根）明显轻于对照组（Ⅰ级0根，Ⅱ级0根，Ⅲ级1根，Ⅳ级1根，Ⅴ级8根），差异有统计学意义（Z=3．922，P〈0．005），实验组移植腱最大抗拉力值（17．38±1．73）N明显高于对照组（11．65±1．29）N，差异有统计学意义（t=8．39，P〈0．001）。结论bFGF与维生素C复合降解膜可以促进成纤维细胞增殖和胶原纤维合成，提高肌腱生物力学性能，防止肌腱粘连。
Objective To investigate the feasibility of preventing tendon adhesion by using bFGF/ vitamin C composite blodegrodabte membrane. Methods Sixty Newzerland rabbits were divided into experimental and control groups randomly. After the animal model was established, the tendon autografts were encapsulated with the bFGF/vitamin C composite biodegradable membrane in experimental group, while no tendon autografts encapsulation in control. Three weeks after surgery, regular HE staining and AgNO3 staining were performed to observe the fibroblast nuclei and vitamin C. The quantity of collagen fibrils was measured by Luzex-F Image Analyzer. Eight weeks after surgery, the peritendinous adhesion and the maximum tensile load were analyzed. Results At 3 weeks after surgery, the numbers of vitamin C granules, fibroblast nuclei and collagen fibrils in the experimental group were significantly more than those in the control group（ t = 11.78 ,P 〈 0. 001 ）. At 8 weeks after surgery, the peritendinous adhesion in the experimental group was significantly slighter than that in the control group（Z =3. 922,P 〈0. 005） ,and the maximum tensile load in the experimental group was significantly higher than that in the control group （ t = 8. 39, P 〈 0. 001 ）. Conclusion bFGF/vitamin C composite biodegradable membrane can stimulate the proliferation of fibroblasts and synthesis of collagen fibrils, improve the biomechanical property of tendon autografts and prevent the tendon adhesion.
出处
《中国综合临床》
2011年第9期904-907,共4页
Clinical Medicine of China
基金
辽宁省科学技术计划项目（2009225009）
辽宁省自然科学基（20082200）
沈阳市科学技术计划项目（071280）
沈阳医学院科学研究基金（20111010）
