摘要
目的建立HPLC内标法测定南方红豆杉不同部位中紫杉醇的含量。方法以地西泮作为内标,色谱柱采用Hypersil ODS2(250 mm×4.6 mm,5μm)柱,流动相为乙腈-甲醇-水(27:36.5:36.5),流速:0.8 ml/min,检测波长:277 nm,柱温:30℃。结果紫杉醇的质量浓度在3.125～100μg/ml(r=0.9995)时,线性关系良好。平均回收率为97.4%(RSD=0.85%)。南方红豆杉根中紫杉醇含量最高,其次为皮,叶和木质含量较少。结论使用内标法进行定量,科学合理、准确可靠,可作为红豆杉原药材或含有红豆杉的中成药进行紫杉醇含量测定的分析方法。
Objective To establish an internal standard HPLC method for the determination of paclitaxel in different parts of Taxus wallichiana var. mairei. Methods Diazepam was used as an internal standard. The determination by HPLC was carried out using a Hypersil ODS2 column （250 mm x4.6 mm, 5 /μm）, with the mobile phase of acetonitrile- metha- nol-water （27： 36.5： 36.5） at a flow rate of 0.8 InL/min and at the detection wavelength of 277 nm. The temperature was set at 30~C. Results The calibration curve was linear in the range of 3. 125 - 100 μg/ml（r =0.9995） for paclitaxel. The mean recovery was 97.4% and RSD was 0.85% （n =6）. Paclitaxel content in was the highest the root, follawed by the bark, leaf and xylon. Conclusion This method can be applied in quantitive determination of paclitaxel with accuracy and reliability.
出处
《军事医学》
CAS
CSCD
北大核心
2011年第8期624-626,共3页
Military Medical Sciences
基金
国家"十一五"重大专项(2009ZX909304-004)
