摘要
为测定不同种类的红豆杉中紫杉醇的含量,采用ZORBAX SB-C18为分析柱,流动相为甲醇-乙腈-水=24:37:39,流速0.8mL/min,检测波长227nm。结果:紫杉醇在0.21019μg～2.10187μg(r=1.0000)范围内呈良好线性关系,平均回收率为96.4%,RSD为1.3%。结论:不同种类的红豆杉中紫杉醇的含量有一定的差异,云南红豆杉、南方红豆杉、曼地亚红豆杉、加拿大红豆杉及欧洲红豆杉中紫杉醇平均含量分别为0.034%、0.023%、0.041%、0.020%、0.024%。该次实验采用的方法简便快捷、灵敏度高、重现性好、准确可行,适用于紫杉醇原料的质量控制。
This study is aimed at determining the content of taxol in different Taxus species. ZORBAX SB-C18 column was used. The mobile phase was methanol-acetonitrile-water=24:37:39 at a flow rate of 0.8 mL/min. The detection wavelength was at 227 nm. The linear relationship of peak area was good when injection volume of Taxol was in the range of 0.21019μg-2.10187 μg(r=1.0000). The average recovery was96.4%and RSD was 1.3%. The content of taxol in different Taxus species was different, and the average taxol content in the five species were 0.034%, 0.023%, 0.041%, 0.020% and 0.024%, respectively. The method adopted in this experiment is simple, fast, sensitive, reproducible, accurate and feasible, and it is suitable for the quality control of paclitaxel raw materials.
作者
赵鑫童
闭帅珠
唐洪节
密昌
卞家亭
ZHAO Xin-tong;BI Shuai-zhu;TANG Hong-jie;MI Chang;BIAN Jia-ting(Yunnan Yunce Quality Testing Co.,Ltd.)
出处
《中国标准化》
2018年第A01期222-225,共4页
China Standardization
