摘要
背景:骨腱结合部组织结构复杂,损伤愈合过程相对缓慢,其愈合过程中的关键在于促进特征性结构纤维软骨移行带的修复。目的:通过组织学方法对腱骨结合部愈合进行评估。方法:18周龄新西兰大白兔随机分为两组。骨膜组将骨膜游离并植入兔髌骨部分切除模型中骨-肌腱结点中,对照组不植入骨膜。术后4,8,12周行组织学染色观察,对比Safranin’O染色阳性染色的积分吸光度值。结果与结论:组织学检查显示骨膜组术后4,8周骨-肌腱结合部组织愈合明显,以从松质骨再生和骨-肌腱愈合接点纤维软骨带的再生为特征,较对照组迅速,提示早期骨膜组恢复较对照组迅速。说明骨膜可以促进骨-肌腱结合部位细胞增生,增加细胞基质合成,促进新生骨和纤维软骨移行带形成,促进其愈合。同时,组织学检测可以很好地判断腱骨结合部的愈合。
BACKGROUND：Wound healing of bone-tendon junction is relatively slow due to complex structures.Repair of fibrocartilage transitional zone plays an important role in bone-tendon junction healing.OBJECTIVE：To explore effects of periosteum on healing of bone-tendon junction via histological methods.METHODS：18-weeks-old New Zealand white rabbits were randomly divided into 2 groups.The periosteum group was planted into by periosteum while the control group was only operated.Histological staining was performed at the 4,8 and 12 weeks after operation,the absorbance value of Safranin＇O positive staining was compared between two groups.RESULTS AND CONCLUSION：Histological examination showed the bone healing was obviously in the periosteum group at 4 and 8 weeks after operation,which characterized by trabecular bone expansion from the remaining patellar and regeneration of fibrocartilage zone at the patellar tendon-patellar healing junction.The result means that the tissue healing of the periosteum group was quicker than that of the control group.Periosteum could accelerate cellular proliferation,matrix synthesis,new bone and fibrocartilage transitional zone formation and accelerate bone-tendon junction healing.Simultaneously,histological examination can be a good judgment for detecting bone-tendon junction healing.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第15期2677-2680,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金面上项目(30973067)
广东省医学科研基金项目(A2008530)~~
