摘要
目的研究1年以内感染HIV的感染者（早期感染者，EHI）体内CD4^＋CD25＋Foxp3＋调节性T淋巴细胞水平及其与疾病进展相关性。方法随机选取51例HIV感染者，依据感染时间及CD4T淋巴细胞水平分为3组：EHI组30例、HIV组15例、AIDS组6例，20名健康人作为对照组，各组对象的年龄、性别具有可比性。用EDTA抗凝管采集全血，应用FACSAria流式细胞仪及Foxp3染色试剂盒，检测外周血单个核细胞CD4^＋CD25＋Foxp3＋调节性T淋巴细胞表达水平，分析EHI者及全部HIV感染者CD4^＋CD25＋Foxp3＋调节性T淋巴细胞表达水平与CD4＋T淋巴细胞数量、病毒调定点、病毒载量及淋巴细胞活化水平间的相关性。结果健康对照组、EHI组、HIV组及AIDS组CD4^＋CD25＋Foxp3＋T淋巴细胞百分率逐级上升，其中EHI组CD4^＋CD25＋Foxp3＋淋巴细胞百分率[3．79（2．11—5．43）％]低于AIDS组[8．09（4．90-8．90）％]，差异有统计学意义（Z=-2．29，P=0．022）；EHI组CD4cD去Foxp3’T淋巴细胞百分率与病毒调定点正相关（r=0．479，P=0．038），与CD4T淋巴细胞计数呈负相关（r=-0．455，P=0．011），与CD3＋HLA’T淋巴细胞呈正相关（r=0．533，P=0．002）。结论中国EHI者CD4^＋CD25＋Foxp3＋调节性T淋巴细胞百分率高与高病毒调定点及低CD4＋T淋巴细胞数量相关，提示CD4^＋CD25＋Foxp3＋调节性T淋巴细胞是加速HIV感染早期疾病进展的因素之一。
Objective To study the alternations of regulatory T cells in early HIV infected patients and its association with disease progression. Methods Fifty-one untreated HIV infected patients were enrolled and divided into 3 groups according to their infection time and CD4＋ T cell levels （30 early HIV infected patients, 15 typical progressors, 6 AIDS patients）. Twenty normal controls were enrolled. There were no significant differences between the age and sex among four groups. Blood was drawn by venipuncture from each subject in EDTA tubes and the levels of CD4^＋CD25＋Foxp3＋ regulatory T cells were detected by FACSAria flow-cytometry. Spearman correlation was used to detect association between CD4^＋CD25＋Foxp3＋ regulatory T cells and the absolute CD4＋ T cells, viral load and activation of T cells. Results The levels of CD4^＋CD25＋Foxp3＋ regulatory T cells showed the tendency of increasing tendency from normal control to early HIV infected patients, asymptomatic HIV infected patients and AIDS patients. Early HIV infected patients was significantly lower than that in AIDS group [3.79（2. 11 -5.43）% vs 8.09（4. 90 -8.90）% , Z = -2. 29, P = 0. 022 ]. The levels of CD4^＋CD25＋Foxp3＋ Treg cells were associated with viral set point （ r = 0. 479, P = 0. 038） and inversely associated with CD4 T cells （ r = - 0. 455, P = 0.011 ） and closely associated with HLA-DR expression on CD3 T cells （ r = 0. 533, P = 0. 002）. Conclusions The ratio of CD4^＋CD25＋Foxp3＋ regulatory T cells of early HIV infected patients was significantly increased and associated with viral set point and CD4 T cell counts, which indicate that alternation of regulatory T cell may be an important factor contributing to the disease progression in early HIV infection.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2011年第8期712-716,共5页
Chinese Journal of Laboratory Medicine
基金
国家科技重大专项课题资助项目（2008ZX100014）01）
辽宁省重点实验室项目（2008S242）
辽宁省医学高峰建设工程专项经费项目（辽卫函字[2010]696号）
辽宁省教育厅高等学校创新团队项目（辽教发2007T184）
