摘要
目的研究低氧诱导因子-1α基因（HIF-1α）第12外显子1790（G—A）单核苷酸多态性与鼻咽癌放疗敏感性的关系。方法收治的经病理明确诊断、完成根治性放疗、随访3年以上的189例鼻咽癌患者，根据随访结果分为治愈组135例和复发组54例，利用聚合酶链式反应．限制性片段长度多态性PCR—RFLP技术检测HIF-1α基因第12外显子1790（G→A）的单核苷酸多态性基因型，并分析其基因多态性特征。结果治愈组和复发组HIF-1α基因1790（G→A）单核苷酸多态性的GG、GA和AA基因型频率分别为77．04％、20．74％、2．22％和59．26％、38．89％、1．85％。G、A等位基因频率分别为87．4％、13．9％和78．7％、21．3％。鼻咽癌复发组HIF-1α基因1790（G→A）单核苷酸多态性等位基因分布频率高于治愈组（x^2=6．619，P〈0．05）。结论HIF-1α基因1790（G→A）多态性可能与鼻咽癌放射敏感性相关。
Objective To explore the association between the genetic potymorphism of hypoxia inducible factor 1α（HIF-1α） G1790A and the radiosensitivity of nasopharyngeal carcinoma. Methods A total of 189 patients with nasopharyngeal carcinoma treated with radical radiotherapy were followed-up for 3 years. The patients were divided into cured group with 135 cases and recurrence group with 54 cases by clinical follow-up results. PCR-RFLP was used to determine the mononucleetide genotypes of HIF-1α G1790A. Results The observed genotype frequencies of HIF-1α gene 1790 （G→A） for GG, GA and AA were 70. 04% , 20.74% , 2.22% in cured group and 59.26% , 38.89% , 1.85% in recurrence group, respectively. The allele frequencies for G and A were 87.4%, 13.9% in cured groups and 78.7%, 21.3% in recurrence group, respectively, without significant difference in distribution of allele frequencies between the two groups （X^2 = 6. 919, P = 0. 077）. Conclusions The genetic polymorphisms of HIF-1α G1790A might be related with the radiosensitivity of nasopharyngeal carcinoma.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2011年第4期430-432,共3页
Chinese Journal of Radiological Medicine and Protection
基金
基金项目：福建省自然科学基金（2007J0284）
关键词
低氧诱导因子
单核苷酸多态性
鼻咽癌
放射敏感性
Hypoxia inducible factor 1α
Single nucleotidepolymorphism
Nasopharyngeal carcinoma
Radiosensitivity
