摘要
目的探讨缺氧条件下电离辐射对鼻咽癌细胞株CNE-1中缺氧诱导因子HIF—1α及P53表达的影响。方法将CNE-1鼻咽癌细胞分为空白对照组、单纯照射组和缺氧照射组。单纯照射组：按照照射率4Gy／min，共8Gy的剂量利用x射线照射细胞；缺氧照射组：氯化钴处理细胞模拟缺氧条件，缺氧一定时间后，按照与单纯照射组相同的剂量照射细胞。MTT法分析细胞存活分数，免疫荧光技术分析两组鼻咽癌细胞中的HIF-1α蛋白表达及分布情况，流式细胞仪定量检测HIF-1α和P53的蛋白表达。结果（1）MTT检测结果：细胞存活分数排列顺序为：对照组〉缺氧加照射组〉单纯照射组（P〈0．01）；（2）HIF-1α蛋白表达情况：免疫荧光显示鼻咽癌细胞HIF-1α蛋白的表达，在缺氧加照射组主要定位于细胞浆中，而另二组未见显示，流式细胞仪示缺氧加照射组HIF—1α明显高于单纯照射组（P〈0．01），单纯照射组与对照组差异无统计学意义（P〉0．05）；（3）P53蛋白表达变化情况：缺氧加照射组〉单纯照射组〉对照组（P〈0．05）。结论鼻咽癌细胞缺氧条件下HIF-1α可能通过P53发挥重要的保护作用，从而降低实体瘤中内部癌细胞对辐射的敏感性。
Objective To investigate the effect of ionization to the expression of HIF-1α and p53 of nasopharyngeal carcinoma （NPC） in anoxia condition. Method Nasopharyngeal carcinoma cell line CNE-1 was divided into three groups, control group, radiation group, and hypoxia plus radiation group. The cell viability was analyzed by MTT method. Immunofluoreseent technique was performed to determine the expression location of HIF-1α in CNE-1. And the expression of HIF-1α and P53 protein were measured with flow cytometry. Restilts The results of MTr detection showed that the survival fraction of the control group was the highest. The minimum survival fraction was found in the radiation group （ P 〈 0.05 ）. There was no HIF-1α protein expression in cytoplasm except the radiation plus hypoxia group. With the flow cytometer technique, the expression of HIF-1 ctprotein in the radiation plus hypoxia group was higher than that in the radiation group. No statistical significance was found between the radiation group and the control group. When detecting the expression of P53 protein with the same technique, the expression of P53 was highest in the hypoxia plus radiation group and lowest in the control group （ P 〈 0. 05）. Conclusion The expression of HIF-1α inducted by Hypoxia can prevent NPC cell from the damages of radiation. The HIF-1α decreases the radiosensitivity through inducing p53 expression in NPC CNE-1 cell line.
出处
《中国医师杂志》
CAS
2009年第2期188-191,共4页
Journal of Chinese Physician
