摘要
目的:比较葛根散等3首方剂对急性酒精性肝损伤小鼠肝细胞凋亡的干预效果,探讨3首方剂防治酒伤的部分作用机制。方法:将小鼠随机分为正常对照组、模型对照组、各给药大、中、小剂量组,制备急性酒精性肝损伤模型,给药组以中药水煎剂灌胃,对照组代以蒸馏水,检测肝组织病理学改变、肝细胞凋亡指数、Bcl-2、Bax蛋白的表达。结果:与正常对照组比较,模型对照组小鼠肝组织脂肪变性、肝细胞凋亡指数及肝细胞Bax阳性表达均明显升高(P<0.01),肝细胞Bcl-2阳性表达明显降低(P<0.01)。与模型对照组比较,葛根散和葛花解酲汤在一定剂量下都能显著减少小鼠肝组织脂肪变性、肝脏凋亡指数和Bax蛋白的表达(P<0.01),显著增加小鼠肝细胞Bcl-2蛋白的表达(P<0.01);石膏汤在一定剂量下能显著减轻小鼠肝组织脂肪变性(P<0.01),但对小鼠肝脏凋亡指数及Bcl-2、Bax的表达无影响,与葛根散比较具有显著差异(P<0.05)。结论:葛根散和葛花解酲汤能减轻肝细胞的变性、坏死,减少细胞凋亡,可能与其解酒机制有关。
Objective： To compare the interventional effects of three anti-inebriation recipes on apoptosis in acute alcoholic liver injury mouse, and study the mechanism of three recipes on alcoholism. Methods： Mice were randomly divided into normal control group, model control group, each drug group in high dosage, middle dosage, and low dosage. After the models of acute alcoholic liver injury mice were established, every drug group was treated with Chinese herb decoction by intragastric gavage, and control groups were administered with distilled water instead of drugs. Then detect the pathological changes, hepatocyte apoptosis index, and the expression of Bcl-2 and Bax in hepatic tissue. Results： The liver steatosis score, hepatocyte apoptosis index, and the expression of Bax in model control group mice were significantly elevated and the expression of Bcl-2 was significantly declined compared with those in normal control group （P〈0.01）. In a certain dose, Pueraria Powder and Gehua Jiecheng Decoction could significantly reduce the liver steatosis score, hepatocyte apoptosis index, and the expression of Bax and elevate the expression of Bcl-2 compared with model control group （P〈0.01）. Gypsum Soup could significantly reduce the liver steatosis score （P〈0.01）, but could not affect hepatocyte apoptosis index and the expression of Bcl-2 and Bax.There were conspicuous differences compared with Pueraria Powder （P〈0.05）. Conclusion： Pueraria Powder and Gehua Jiecheng Decoction could reduce liver cell degeneration and necrosis and hepatocyte apoptosis. It may be relevant to their anti-inebriation mechanisms.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第9期2046-2048,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
贵州省科技厅基金项目(黔科合J字[2008]2290)
贵州省省长专项资金项目(黔科合专字[2008]64号)~~
关键词
葛根散
石膏汤
葛花解酲汤
细胞凋亡
实验小鼠
Pueraria Powder
Gypsum Soup
Gehua Jiecheng Decoction
Apoptosis
Experiment mouse
