摘要
目的研究菟丝子多糖含量测定的方法。方法制备菟丝子精制多糖,采用13C-NMR检测其纯度;PMP衍生化法结合高效液相色谱法检测精制前后多糖的单糖组成。以精制多糖测得粗多糖对葡萄糖的换算因子,并利用苯酚硫酸分光光度法测定黑龙江、山东及内蒙古菟丝子多糖的含量。结果精制多糖基本不含其他杂质,菟丝子多糖精制前后单糖组成无明显变化。多糖供试液在6 h内显色稳定,重现性好,平均加样回收率为99.4%,RSD=3.02%(n=6),测得黑龙江、山东、内蒙古菟丝子中多糖含量分别为8.02%、23.20%、10.72%。结论引入换算因子来校正多糖含量测定方法,结果更客观准确。
Objective To study the method for quantitative determination of polysaccharides in Tusizi（Semen Cuscutae）.Methods The refined polysaccharides in Tusizi were prepared and the purity was detected by using 13C-nuclear magnetic resonance（13C-NMR）.The monosaccharide composition of polysaccharides was detected by using PMP-pre-column derivatization and HPLC before and after refining.The conversion factor of crude polysaccharides to glucose was obtained with refined polysaccharides,and the content of polysaccharides was determined by using phenol-sulfuric acid spectrophotometry in the samples of Tusizi produced from Heilongjiang,Shandong and Inner Mongolia.Results The refine polysaccharides of Tusizi had no other impurities,and monosaccharide composition of Tusizi polysaccharides had no obvious changes before and after refining.The colouration of polysaccharides test solution was stable with a good repeatability within 6 hours.The average recovery was 99.4%（RSD=3.02%,n=6）.The polysaccharides content in Tusizi produced from Heilongjiang,Shandong and Inner Mongolia was,respectively,8.02%,23.20% and 10.72%.Conclusion The results are more objective and accurate when conversion factor is brought in for correcting the quantitative determination of polysaccharides.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2011年第8期548-551,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
北京中医药大学校级课题
关键词
菟丝子
多糖
含量测定
精制
Tusizi（Semen Cuscutae）
polysaccharides
quantitative determination
refining
