摘要
通过RNA亲和层析(RNA affinity chromatograph),4种可能与人PSF(human polypyrimidinetract-binding protein-associated splicing factor,hPSF)蛋白结合的长非编码RNA(1ong non-coding RNA,lncRNA)片段在人黑色素瘤细胞yusac细胞核RNA文库中被筛选得到.它们分别定位于人内源性逆转座子L1PA16、MER11C、非编码基因MALAT-1以及一个未知基因(unknown gene).紫外交联分析(UV cross-linking assay)证明在体外条件下这4种lncRNA片段均能与PSF蛋白结合;RNA免疫沉淀(RNA immunoprecipitation,RNA-IP)进一步证实在体内条件下这4种lncRNA均能与hPSF发生相互作用.最后,半定量RT-PCR表明,未知lncRNA特异高表达于人黑色素瘤细胞,其余3种lncRNA在多种人类肿瘤细胞中的丰度均较正常细胞明显升高.
Four hPSF （human polypyrimidine tract-binding protein-associated splicing factor）-binding lncRNA （long non-coding RNA） fragments were isolated by RNA affinity chromatograph from a nuclear RNA library of human yusac melanoma cell line. They map to retrotransposon L1PA16 and MERllC, non-coding gene MALAT-1 and an unknown gene, respectively. UV cross-linking assay confirmed that the four lncRNAs bind to hPSF in vitro. RNA-immunoprecipitation （RNA-IP） demonstrated that they also form lncRNA/hPSF protein complexes in vivo. A screen of 9 human tumor cell lines by semi-quan titative RT-PCR shown that the lines. The other three lncRNAs cells. unknown lncRNA was exclusively expressed in human melanoma cell were also overexpressed in human tumor cells in contrast to normal
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第4期910-916,共7页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金(31000579)
海南省自然科学基金(310045)
