摘要
PCR技术是现代分子生物学最基础的技术之一,在实现扩增效果的同时如何缩短PCR的实验时间成为实验工作者亟待解决的问题。本文从高速PCR的概念入手,阐述了实现高速PCR仪器技术的原理、优势,及可能的应用。指出了高速PCR技术的核心在于,不仅要提高PCR仪器的升降温速率,更重要的是提高PCR样品温度与金属样品槽的一致性,从而达到缩短变性,退火,延伸等停留时间的目的,进而大大的缩短PCR时间。同时也指出,高速PCR对提高反应特异性带来的好处等。
PCR technology has been one of the basic and key technologies of Modern Molecular Biology.How to shorten PCR time is a question badly needed to be solved on the premise of PCR amplification effect.In the paper we describe the concept,principle,advantage and application of rapidPCR technology which can highly increase the PCR speed.As addressed in the paper,the Key of rapid PCR is not only to increase the heating and cooling rate of PCR machine,more important,it relays on reducing the temperature difference between the PCR module and sample solution,so that the holding time of denaturation,annealing,elongation could be shorten,and the whole PCR time could be shorten dramatically.While,rapid PCR could also lead to another benefit to improving the specificity of PCR
出处
《现代科学仪器》
2011年第4期128-130,共3页
Modern Scientific Instruments
关键词
PCR
高速PCR
自适配技术
产物特异性
PCR
rapid PCR
Self-Adapting-Container technology
product specificity
