摘要
文章设计了内部不含温度传感元件、加热元件的低成本微反应槽聚合酶链式反应(PCR)芯片,研制了宏观集中控制与微观分散控制有机结合的PCR芯片阵列温度控制系统。宏观集中控制装置以水为传热媒质,通过特制换热器给芯片提供聚合酶链式反应所需的基本温度,在换热器每个等温区选择一个芯片充满水作为测控样板并插入微型热电偶。在柔性印刷电路板上形成与芯片阵列对应的微型加热器阵列,根据测控样板的实测数据对各芯片进行分散的温度补偿,并采用串级控制策略实现微加热器阵列与换热器之间的良好配合。
A kind of low cost micro - reactor PCR chips is designed in which there are no temperature sensor and heater, and a temperature control system to the array of this kind of chips is developed. The control system is composed by a unitized microscopic control subsystem and a distributed microcosmic control subsystem. The temperature that PCR chips needed is controlled approximately by unitized microscopic subsystem in which water as heat medium is pumped through a special heat exchanger. The heat exchanger can be divided into several areas and the temperature deference in each area can be neglected. There is a measurement and control model chip in each area and a micro - thermocouple is set in it. An array of miniature heaters corresponding to the array of PCR chips is made in a flexible printed circuit board that provides temperature compensation to every PCR chip according to the feedback signal of model chip. To be composed a cascade control system, the miniature heaters cooperate together with the heat exchanger to control the temperature of every chip quickly and accurately.
出处
《机电一体化》
2006年第4期19-23,共5页
Mechatronics
基金
国家自然科学基金资助项目(90307003)
关键词
聚合酶链式反应
微机电系统
工业控制计算机
串级控制
polymerase chain reaction （PCR） microelectromechanical systems （MEMS） industrial control computer cascade control
