摘要
建立了测定左旋苯甘氨酸乙基邓钾盐含量的高效液相色谱方法。采用Thermos C18色谱柱,流动相为0.01 mol/L磷酸盐缓冲液(含0.2%(体积分数)三乙胺,85%(体积分数)磷酸,调节pH值为3.0)-乙腈(体积比为5∶95)为流动相,检测波长为220 nm。样品在pH值为2.0条件下水解后,以水解产物苯甘氨酸为外标,计算左旋苯甘氨酸乙基邓钾盐的含量。左旋苯甘氨酸乙基邓钾盐质量浓度在2～20 mg/mL范围内线性关系良好(r=0.999 9),回收率为96%～98%。
Liquid chromatography method of high performance for the determination of l-glycine ethyl benzene Deng sylvite is established.C18 column is adopted with the mobile phase of 0.01 mol/L phosphate buffer（containing 0.2% triethylamine,adjusted pH to 3.0 by 85% phosphoric acid）-acetonitrile＇s（5∶95,V/V）.The detection wavelength is 220 nm.L-glycine ethyl benzene Deng sylvite hydrolyzed at pH 2.0,then phenylglycine is used as the external standard to calculate the content.A good linearity is shown in the range of 2 ~ 20 mg/mL（r=0.999 9）,and the recoveries are 96%~98%.
出处
《河北工业科技》
CAS
2011年第5期288-290,294,共4页
Hebei Journal of Industrial Science and Technology
