摘要
目的在细胞水平上研究脂多糖(lipopolysaccharide,LPS)激活血清补体对内皮细胞的作用和影响。方法研究LPS激活血清补体的作用方式和特点,观察LPS激活补体对内皮细胞表达黏附分子和凋亡的影响。采用ELISA检测内皮细胞释放P-selectin、E-selectin和ICAM-1的变化,化学发光法检测Caspase-3/7活化情况,SRB法检测LPS激活补体对内皮细胞生长的影响。结果 LPS能够激活血清补体,这种激活呈现量效、时效性。LPS激活血清补体可诱导内皮细胞瞬时明显释放P-selectin,E-selectin和ICAM-1表达也明显上调,同时可导致Caspase-3/7活化。在本实验条件下,LPS激活血清补体对内皮细胞生长未产生抑制。结论 LPS激活血清补体可损伤内皮细胞,导致内皮细胞明显表达黏附分子以及发生凋亡。
Aim To investigate the effects of lipopoysaccharide-activated complement on endothelial cells. Methods The manner of LPS activating complement was characterized. Then the effects of LPS-ac- tivated complement on endothelial cells were observed. P-selectin, E-selectin, and ICAM-1 were determined by ELISA. The activity of Caspase-3/7 was measured by chemiluminescence method. The growth of endothelial cells was measured by SRB method. Results LPS activated complement in a doseand time-dependent manner. The incubation mixture of LPS and comple-ment significantly induced the release of P-selectin, E- selectin, and ICAM-1. The activity of Caspase-3/7 was also significantly increased. But the incubation mixture nearly showed no inhibition on the growth of endothelial cells. Conclusions LPS-activated com- plement can injure endothelial cells, and significantly up-regulate the expression of adhesion molecules and apoptosis.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2011年第9期1245-1249,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No31060124)
国家重点基础研究发展计划(973计划)资助项目(No2009CB526512)
贵州省优秀科技教育人才省长专项资金资助项目(No黔省专合字2006-65号)
贵州省优秀青年科技人才项目(No黔科合人字2005-0510号)
关键词
脂多糖
补体
内皮细胞
黏附分子
凋亡
补体激
活
眼镜蛇毒因子
lipopolysaccharide
complement
endothe-lial cell
adhesion molecule
apoptosis
. complementactivation
cobra venom factor
