摘要
采用大孔树脂对匙羹藤总皂苷进行分离富集。考察了5种树脂对匙羹藤总皂苷的静态吸附效果并筛选出D101树脂用于分离富集匙羹藤总皂苷,通过动态吸附性能考察,确定了D101树脂固定床分离富集匙羹藤总皂苷的工艺条件,在ρ(总皂苷)=5 mg/mL,pH=6,料液以1.0 mL/min的流速通过D101树脂固定床进行吸附,分别用去离子水和φ(乙醇)=10%溶液洗脱杂质后,再用φ(乙醇)=75%溶液洗脱匙羹藤总皂苷。经过分离富集后,w(总皂苷)由粗浸膏的25.33%提高到51.07%,总皂苷的回收率为83.26%。
Total saponins of Gymnema sylvestre were separated and enriched with macroporous resins.The adsorption capacity and adsorption ratio of five kinds of adsorption resins for total saponins in extraction of Gymnema sylvestre were investigated.The results of saturated static adsorption of different types of resins showed that D101 was suitable for the separation of total saponins.Further,the concentration and pH of total saponins of Gymnema sylvestre in solution,flow rate,the concentration of the elution reagent were optimized.The results showed that the purity of total saponins of Gymnema sylvestre was raised to 51.07% from 25.33% and the recovery was 83.26%.Total saponins of Gymnema sylvestre was enriched effectively.
出处
《化工科技》
CAS
2011年第4期7-10,共4页
Science & Technology in Chemical Industry
基金
广西壮族自治区科技攻关项目(桂科攻0718002-4)
广西大学校级项目(X081039)
关键词
匙羹藤
总皂苷
大孔树脂
富集纯化
Gymnema sylvestre
Total saponins
Macroporous resin
Enrichment and purification
