摘要
目的:观察二草清肝汤对小鼠内毒素血症急性肝损伤的影响及Toll样受体4(toll-Likereceptor4 TLR4)表达的影响。方法:取BALB/c小鼠120只,随机分为正常对照组、内毒素肝损伤模型组及二草清肝汤大、小剂量组,各30只。二草清肝汤大、小剂量组分别予二草清肝汤6mg/kg、3mg/kg预防性灌胃,正常对照组与模型组给予等量生理盐水灌胃,1天1次。12天后除正常对照组外,另三组给予D-氨基半乳糖(D-Gal)和LPS腹腔注射,观察注射后1、2、4、6、12、24h时各组小鼠光镜下肝组织的病理变化,采用全自动生化分析仪检测血浆丙氨酸氨基转移酶(ALT)水平,酶联免疫吸附法检测血清IL-12、TNF-α浓度,免疫组织化学法检测TLR4表达水平,RT-PCR检测肝组织TLR4 mRNA表达水平。结果:二草清肝汤大、小两个剂量组各个时间点血ALT值、血清IL-12、TNF-a水平均低于同时间点的模型组(P<0.05或P<0.01),TLR4 mRNA水平也明显低于同时间点的模型组,差异均有统计学意义(P均<0.05)。但二草清肝汤大、小剂量组间比较差异无统计学意义(P>0.05)。结论:预防性给予二草清肝汤,可抑制内毒素血症肝损伤小鼠肝脏炎症反应,其机制可能与下调LPS识别受体TLR4表达,并进而抑制下游炎症因子分泌有关。
Objective： To observe the effects of Ercao Qinggan decoction on liver inflammatory reaction of mice with endotoxemia and the expression of toll-like receptor 4（TLR4）.Methods： A total of 120 BALB/c mice were randomized into 4 groups： normal control group,model group,high-dose of Ercao Qinggan decoction group,and low-dose of Ercao Qinggan decoction,30 in each.The high dose of 6 mg/kg and the low dose of 3 mg/kg of Ercao Qinggan decoction were intragastrically administered once a day in the 2 experimental groups,respectively,and the same volume of saline to the normal control and model groups.After 12 days of pretreatment,D-Gal and lipopo1ysaccharido were intraperitoneally injected to the modal and experimental groups.In 1,2,4,6,12,and 24 h after injection,tissue and serum samples were taken to observe the pathological damages,to measure the level of alanine aminotransferase（ALT）,to determine the amounts of IL-2 and TNF-α by ELISA,and to detect the expression of TLR4 by immunochemistry and TLR4 mRNA by RT-PCR.Results： At each time point,the level of ALT,the amounts of IL-2 and TNF-αin 2 experimental groups were lower than those of the model group（P0.05 or P0.01）,and the same change was observed in the expression of TLR4 mRNA（P0.05）.But no difference of these index was noted between the high-dose and low-dose of Ercao Qinggan decoction pretreated groups（P0.05）.Conclusion： Pretreatment with Ercao Qinggan decoction can reduce the inflammatory reaction in the liver of mice with endotoxemia,and its underlying mechanism could be down-regulating the expression of TLR4 and restraining secretion of downstream inflammatory factors.
出处
《浙江中西医结合杂志》
2011年第8期524-527,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
温州市医药卫生科研项目基金(No.2010B051)
