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小麦新品系2-26中抗白粉病基因的遗传分析和RAPD标记

GENETIC ANALYSIS AND RAPD MARKERS OF POWDERY MILDEW RESISTANCE GENE IN WHEAT LINE 2-26
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摘要 由小麦白粉病菌引起的白粉病是世界上很多小麦种植区的主要病害之一。本研究对稳定抗白粉病品系2-26中的抗病基因采用常规遗传方法进行了分析。结果表明,2-26中存在一对显性抗白粉病基因,暂命名为Pm2-26。运用RAPD方法对白粉病抗感亲本和抗感池进行DNA多态性分析,获得2个紧密连锁的RAPD标记(SBSC2和SBSI20),为进一步转化为稳定可靠的SCAR标记提供了基础。 Powdery mildew,caused by Blumeria Graminis f.sp.tritici,is one of the most important damaging diseases in many regions of the world.In present study,genetic analysis of resistance for powdery mildew in a stable wheat line 2-26,which was derived from the hybrid progeny between Triticum durum-Dasypyrum villosum amphiploid and common wheat,was carried out.The result indicated the powdery mildew resistance in line 2-26 is controlled by a single dominant gene and temporarily named Pm2-26.Molecular markers and bulked sergeant analysis were used to characterized the powdery mildew resistance gene.Two RAPD markers(SBSC2 and SBSI20) were found tightly linked to the resistance gene in line 2-26.These results provided a basis to further transfer into stable SCAR marker in future.In addition,the gene origin in line 2-26 and relationships with other resistance genes were also discussed in this paper.
出处 《核农学报》 CAS CSCD 北大核心 2011年第4期679-683,共5页 Journal of Nuclear Agricultural Sciences
基金 四川省教育厅重点项目(09ZA085)
关键词 小麦 白粉病 抗性基因 分子标记 wheat powdery mildew resistance gene molecular marker
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