摘要
目的:考察不同炼制温度和用量的羊脂油制淫羊藿对肾阳虚大鼠的影响。方法:采用皮下注射皮质酮(CORT)建立肾阳虚大鼠模型,以淫羊藿总黄酮为阳性对照,以症状体征、动物抓力、组织病理学检查、血清睾酮含量等为评价指标,3种制淫羊藿(羊脂油炼制温度与加入量分别为250℃,30%;120℃,30%;120℃,20%)和生淫羊藿经水提取灌胃给药,考察不同样品对肾阳虚大鼠的药理作用。结果:3个炮制品均能不同程度地增强生品的温肾壮阳作用,其中以120℃,30%的制淫羊藿增强效果最佳。其作用机制可能与改善肾阳虚证大鼠下丘脑-垂体-肾上腺-胸腺(HPAT)轴病理改变和功能抑制状态有关。结论:辅料羊脂油的炼制温度影响辅料质量,进而影响饮片药效。本试验结果为进一步阐明制淫羊藿炮制原理及建立炮制辅料的国家标准提供实验数据。
Objective： To investigate the effects of refining temperatures and amounts of sheep′s oil used in processing Epimedii Folium on Kedney-yang deficiency rats.Method： The corticosterone was subcutaneous injected to establish the kidney yang deficiency rat model.With the temperatures and amounts of sheep′s oil were 250 ℃/30%,120 ℃/30% and 120 ℃/20% respectively,the crude drug and three kinds of prossed Epimedii Folium were extracted by water and used as examined samples while total flavonoid of Epimedii Folium was used as positive control.After examined samples and control samples were intragastirc administrated,the pharmacologic action was analyzed.Result： As compared to crude drug,all of the aqueous extracts of processed Epimedii Folium have stronger effect of warming kidney and enhancing yang,especially the Epimedii Folium processed by sheep′s oil with refining temperatures 120 ℃ and amounts of sheep′s oil 30%.Its mechanism might be related to improving the insufficiency of hypothalamic-pituitary-adrenal-thymus（HPAT） axis suppression.Conclusion： The refining temperature of sheep′s oil can affect the quality of excipients and processed drugs.The results may be useful in explaining the mechanism of Epimedii Folium processing and establishment of pharmaceutical standard of sheep′s oil used as processing excipients.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第16期2250-2254,共5页
China Journal of Chinese Materia Medica
基金
国家科技部科研院所社会公益研究专项(2005D1B1J169-01)
中国中医科学院传承博士后项目(63489)
关键词
羊脂油
制淫羊藿
肾阳虚
炮制辅料
sheep′s oil
processed Epimedii Folium
kidney yang deficiency
processing excipients
