摘要
目的:利用反相高效液相色谱法测定大鼠血浆中羟基红花黄色素A的浓度,分别比较红花提取物及复方脑得生片中羟基红花黄色素A的药代动力学参数差别,探讨复方配伍对羟基红花黄色素A在大鼠体内药动学的影响。方法:以相同剂量的羟基红花黄色素A(50 mg.kg-1)对大鼠分别灌胃给予红花药材提取物和复方脑得生片,采用反相高效液相色谱法测定不同时间点血浆中羟基红花黄色素A的含量,利用3P97软件处理数据,进行药动学模型拟合并计算二者的药代动力学参数。结果:羟基红花黄色素A血浆浓度在0.03～2.56 mg.L-1线性关系良好,血浆中最低检测限和最低定量限分别为10,30μg.L-1。高、中、低浓度样品平均回收率分别为(99.3±1.4)%,(92.8±1.8)%,(98.4±2.0)%。大鼠灌胃给予红花提取物和脑得生片后的药-时曲线均符合二房室模型,主要药动学参数AUC0～t,AUC0～∞,Cmax和tmax在红花提取物和脑得生片各组间均有显著性统计学意义。结论:本实验建立的反相高效液相色谱测定法专属、准确、灵敏,适用于羟基红花黄色素A在大鼠体内的药动学研究。脑得生片中其他配伍药材可以促进羟基红花黄色素A的吸收,提高羟基红花黄色素A的生物利用度。
Objective： To develop a RP-HPLC method for the determination of the concentration of hydroxysafflor yellow A in rat plasma,to study the pharmacokinetics of Carthamus tinctorius extration and Naodesheng tablet,and to investigate the effect of other components on the pharmacokinetics of hydroxysafflor yellow A.Method： The rats were orally treated with Carthamus tinctorius extration and Naodesheng capsule respectively.Blood samples were collected in heparinized eppendorf tube via the oculi chorioideae vein.Plasma was separated by centrifugation at 10 000 r·min-1 for 10 min,and two-times methanol in volume was added to deposit proteins.After centrifugation,the upper liquid was transferred to filter.The concentration of hydroxysafflor yellow A in serum was determined by RP-HPLC.The stationary phase was C18,and methanol-acetonitrile-0.7% orthophosphoric acid（26∶2∶72） was taken as the mobile phase,A UV detector was used at 403 nm.The pharmacokinetic parameters were calculated with 3p97 program.Result： A good linear relationship of hydroxysafflor yellow A was obtained in the range of 0.03 and 2.56 mg·L-1,the lowest limit of determination was 10 μg·L-1,and the lowest limit of quantitation was 30 μg·L-1.The mean recoveries were（99.3±1.4）%,（92.8±1.8）%,（98.4±2.0）% for high,middle,low concentrations of the samples respectively.The plasma concentration-time curves of hydroxysafflor yellow A were fitted with two-compartments model.The AUC0-t,AUC0-∞,Cmax and Tmax of hydroxysafflor yellow A were increased in the Naodesheng group,compared with 50 mg·kg-1 C.tinctorius extract group.Conclusion： The HPLC method was selective,accurate and sensitive.The results indicated that the other herbs improved the absorption of hydroxysafflor yellow A and increased the bioavailability of hydroxysafflor yellow A significantly.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第16期2246-2249,共4页
China Journal of Chinese Materia Medica
基金
江苏大学高级专业人才科研启动基金项目(06JDG042)
关键词
羟基红花黄色素A
红花
脑得生片
药动学
高效液相色谱法
hydroxysafflor yellow A
Carthamus tinctorius
Naodesheng tablet
pharmacokinetics
RP-HPLC
