摘要
雄性SD大鼠27只分为对照组、肥胖组、肥胖伴糖尿病组。测定血皮质酮浓度，行正葡萄糖高胰岛素钳夹实验。采用实时PCR检测各组大鼠肝X受体（LXR）、11β-羟基类固醇脱氢酶1（11β-HSD1）和糖皮质激素受体（GR）mRNA的表达情况。肥胖组和肥胖伴糖尿病组大鼠较对照组具有明显的胰岛素抵抗。与对照组相比，肥胖组和肥胖伴糖尿病组肝LXR mRNA表达均显著升高，11β—HSD1 mRNA表达均显著降低（均P〈0．05）。
Twenty-seven male SD rats were assigned to 3 groups ： control, obese, and obese with diabetes groups. Serum corticosterone was determined and euglycemic-hyperinsulinemic clamp was carried out. Expressions of liver X receptor （ LXR）, 11β-hydroxysteroid dehydrogenase 1 （ 11 β-HSD1 ）, and glucocorticoid receptor （GR） were determined by real-time PCR. Both groups of obese rats were insulin-resistant, with up-regulated LXR mRNA and down-regulated 11β-HSD1 mRNA （ both P〈0. 05 ）.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2011年第8期677-679,共3页
Chinese Journal of Endocrinology and Metabolism
基金
上海市卫生局科研课题基金资助项目（2008063）
