摘要
目的:研究健脾清化方对2型糖尿病(T2DM)大鼠胰岛素抵抗(IR)的影响。方法:采用高脂饮食联合链脲佐菌素(STZ)建立稳定的2型糖尿病大鼠模型,随机分为正常组、模型组、健脾清化方组、吡格列酮组,连续灌胃给药6周,采用高胰岛素-正糖钳夹试验(HEC)、腹腔注射葡萄糖耐量试验(IPGTT)和胰岛素耐量试验(IPITT)综合评价胰岛素抵抗水平。结果:与模型组比较,健脾清化方组IPGTT中60、120min血糖显著下降(P<0.01),药时曲线下面积(AUC)降低(P<0.01),IPITT中各点血糖下降(P<0.01),降糖率显著升高(P<0.01),AUC显著降低(P<0.01);在HEC中葡萄糖输注速率(GIR)显著升高(P<0.01)。结论:健脾清化方可显著改善T2DM大鼠IR,并能降低空腹血糖,提高降糖率,改善糖耐量与胰岛素耐量。
Objective： To study the effects of Jianpi Qinghua Formula on insulin resistance（IR） in rat with type 2 diabetes mellitus（T2DM）. Methods： Fifty Wistar rats were randomly divided into a normal, control, Jianpi Qinghua Formula and pioglitazone groups. The T2 DM rat model was established with streptozotocin（STZ） and high fat diet. All the groups were treated by gavage for six weeks. The level of IR was evaluated by hyperinsulinemic-euglycemic clamp test（HEC）, intraperitoneal glucose tolerance test（IPGTT） and insulin tolerance test（IPITT）. Results： Blood glucose and AUC of Jianpi Qinghua Formula group after 60 min and 120 min were decreased significantly tested by IPGTT（P〈0.01）. Blood glucose at each time point and AUC declined tested by IPITT（P〈0.01）. The hypoglycemic rate increased significantly（P〈0.01）. GIR was increased significantly tested by HEC（P〈0.01）. Conclusion： Jianpi Qinghua Formula could effectively ameliorate IR, decrease fasting blood glucose, increase hypoglycemic rate and improve the glucose tolerance and insulin tolerance of rats with T2 DM.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第10期3239-3242,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
上海市卫生局项目(No.2010X004A)
上海市中医药事业发展三年行动计划项目(No.ZYSNXDCC-YJXYY)
上海市中医重点实验室(No.C10dz2220200)
上海市教委重点学科(第五期)建设项目(No.J50307)
浦东新区卫生系统重点学科群建设项目(No.PWZxkq2010-04)~~
关键词
从脾论治
健脾清化方
胰岛素抵抗
高胰岛素-正糖钳夹
Treating through spleen
Jianpi Qinghua Formula
Insulin resistance
Hyperinsulinemic-euglycemic clamp
