摘要
目的观察封闭式负压引流技术（VAC）治疗兔急性创面的效果，探讨其促进创面愈合的机制。方法将12只新西兰大白兔背部24个急性创面（每只兔2个创面）用随机数字表法分为VAC组和对照组，VAC组给予VAC治疗，负压为120mmHg，共治疗7d；对照组给予常规换药治疗。治疗第3和7天观察创面愈合情况；于创缘取材，采用HE染色观察组织学变化；采用RT—PCR方法检测创缘组织cx43mRNA的表达水平。结果创面大体观察示，VAC组创面较对照组红润、清洁，水肿消退快；镜下可见VAC组上皮较对照组厚，炎症细胞浸润较多；VAC组cx43mRNA表达在治疗第3、7天均明显低于对照组（P〈0．05）。结论VAC可促进急性创面愈合，其机制可能与其促进急性创面炎症细胞浸润，下调组织cx43mRNA的表达有关。
Objective To observe the effects of drainage with vacuum and closure （VAC） on acute wounds, and explore the mechanism of drainage with VAC in promoting wound healing. Methods Twenty-four acute wounds were inflicted on the backs of 12 New Zealand white rabbits （each rabbit two wounds） , and the rabbits were divided into a drainage with VAC group and a control group randomly. The drainage with VAC group was treated with drainage with VAC. The control group was treated with wet saline gauze. The wounds were observed 3 and 7 days after treatment. Patho-morphologi- cal changes in tissues from the compressed area were observed by HE staining. The expression level of Cx 43 mRNA was detected using a RT-PCR. Results At the 3rd and 7th day after treatment, the wounds of the drainage with VAC group were clean, fresh and had less edema compared with those of the control group. Pathomorphological tissue changes were more obvious in the drainage with VAC group. The expression of Cx 43 mRNA in the drainage with VAC group had declined significantly compared with the control group. Conclusion Drainage with VAC can promote inflammatory cell infiltration, down-regulate the expression of Cx 43 mRNA, and promote wound healing.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2011年第8期579-582,共4页
Chinese Journal of Physical Medicine and Rehabilitation
