摘要
目的观察姜黄素对人肝癌细胞SMMC-7721生长抑制和诱导凋亡的影响。方法以不同浓度(10μmol/L、30μmol/L、90μmol/L)的姜黄素作用于体外培养的SMMC-7721细胞48h,MTT法检测姜黄素对细胞的生长抑制率;基于Annexin V-FITC/PI双染的流式细胞方法检测不同浓度姜黄素诱导SMMC-7721细胞的凋亡率;基于AO染色的荧光显微镜方法观察姜黄素对肝癌细胞形态的影响和诱导凋亡的效应。结果 MTT检测显示,姜黄素对SMMC-7721细胞的生长具有抑制作用,呈剂量依赖效应,各实验组与对照组比较均有显著性差异(P<0.05);流式细胞检测结果显示,姜黄素可诱导细胞凋亡,并随着药物浓度的增加细胞凋亡率增加,中剂量组和高剂量组肝癌细胞的凋亡率显著高于对照组(P<0.01);AO荧光检测结果显示,在姜黄素的作用下,细胞数量明显减少,呈现特征性的凋亡形态,凋亡细胞计数显示随着药物浓度增加凋亡细胞增加,提示姜黄素可诱导SMMC-7721细胞凋亡,并呈剂量依赖性。结论姜黄素能抑制SMMC-7721细胞增殖并诱导细胞凋亡,两种作用均具有剂量依赖关系。
Objective To investigate the effects of curcumin on growth and apoptosis of human hepatoma SMMC-7721 cells.Methods The human hepatocellular carcinoma SMMC-7721 cells were treated with 10,30 and 90μmol /L curcumin for 48 hours respectively in vitro.The inhibition rate of SMMC-7721 cells were detected by MTT assay;by Annexin V-FITC/PI double staining analysis,cell apoptotic rate were measured;by AO staining,the morphological and biochemical character changes of apoptosis cells were observed under the culture condition with different concentrations of curcumin.Results MTT method revealed that curcumin could inhibit the proliferation of hepatoma SMMC-7721 cells in a dose dependent manner.Each group had significant difference compared with negative control group（P〈0.05）.Flow cytometry analysis showed that curcumin could induce apoptosis of SMMC-7721,apoptotic rates were increased obviously with the adding up of curcumin concentration.Except low dose group,apoptotic rates of all the other group had extremely significant difference with the control one（P〈0.01）.Result of the AO staining showed that the cell density decreased with the increased of curcumin concentration and a small number of cells appeared obviously apoptotic morphological characteristics.It suggested that Curcumin could induce SMMC-7721 apoptosis in a dose dependent manner.Conclusion Curcumin could inhibit the proliferation of hepatoma SMMC-7721 cells and induce apoptosis in a dose dependent manner.
出处
《湖北中医药大学学报》
2011年第4期5-8,共4页
Journal of Hubei University of Chinese Medicine
基金
湖北省教育厅科学技术研究重点项目(D20101808)
