摘要
对影响里氏木霉(Trichoderma reesei)40359原生质体制备和再生的条件:包括菌龄、水解酶液的种类及浓度、酶解温度、酶解时间、再生培养基的稳渗剂进行了研究。结果表明:当菌龄为12 h,采用2%纤维素酶和2%蜗牛酶混合液,酶解温度30℃,酶解时间2 h,用0.6 mol.L-1蔗糖作再生培养基的稳渗剂,原生质体的形成数为6.14×105个.mL-1,原生质体再生率为10.6%。
The protoplast preparation and regeneration conditions in Trichoderma reesei 40359 were studied.The results showed that the optimal condition for protoplast preparation in Trichoderma reesei 40359 was culture mycelia for 12 h,then enzymelysed for 2 h at 30 ℃ by the mixture solution of snail digestase（2%） and cellulase（2%）.The protoplasts formation number was 6.14×105·mL-1 and the regeneration rate was 10.6% under the optimal condition when 0.6 mol·L-1 surose used as the osmotic stabilizer.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2011年第8期108-112,共5页
Journal of Northeast Agricultural University
基金
哈尔滨市科委留学回国人员项目
关键词
里氏木霉
原生质体
原生质体形成与再生
Trichoderma reesei
protoplasts
preparation and regeneration of protoplasts
