摘要
禾长蠕孢菌(Helminthosporium gramineum f. sp. echinochloae,HGE)是稗草生防潜力菌,为建立HGE的遗传转化体系,研究了菌龄、胞壁降解酶、稳渗剂等对其原生质体制备和再生的影响。结果表明,HGE菌原生质体制备的适宜条件为:26℃下采用1.0%崩溃酶处理菌龄为6h的HGE菌丝体2h,0.7mol/LNaCl为稳渗剂。原生质体再生的适宜条件为:32℃下采用0.5%崩溃酶处理菌龄为16h的HGE菌丝体4h,0.7mol/LNaCl为稳渗剂,R1为高渗再生培养基。
Helminthosporium gramineum f. sp. echinochloae（HGE）is a promising biological control barnyard grass agent. To establish the genetic transformation system of HGE,the optimum conditions including mycelium incubation time,various compounding of enzymes and osmotic stabilizers for protoplast isolation and regeneration from HGE protoplasts were examined. The results indicated that protoplasts were optimally isolated when mycelia of HGE had been cultured for 6 h,and digested with 1% Drislase at 26℃ for 2 h in osmotic stabilizer of 0.7mol·L^-1 NaCl solution. The maximum regeneration ratio was obtained on the R1 regeneration medium when the mycelia of HGE were inoculated after 16 h,and digested with 0.5% Drislase at 32℃ for 4 h in 0.7mol·L^-1 NaCl solution.
出处
《浙江农业学报》
CSCD
北大核心
2010年第1期14-19,共6页
Acta Agriculturae Zhejiangensis
基金
浙江省自然科学基金(Y306180)
中央级公益性科研院所专项资金(2009RG0041)
国家863项目(2006AA10A214)
关键词
禾长蠕孢菌HGE
原生质体制备
原生质体再生
稗草生防
Helminthosporium gramineum f. sp. echinochloae （HGE）
formation of protoplast
generation of protoplast
biological control of barnyard grass
