摘要
目的探讨PTEN、Her-2和Glut-1蛋白在子宫内膜样腺癌(endometrioid adenocarcinoma,EC)发生发展过程中的表达及意义。方法应用免疫组织化学法对40例子宫内膜复杂性增生,44例不典型增生和60例EC组织中的PTEN、Her-2和Glut-1蛋白进行检测。结果 PTEN在复杂性增生、不典型增生和EC表达缺失率分别为42.5%、61.4%和66.7%。在不典型增生与EC间差异无显著性(P>0.05),但EC与复杂性增生间差异具有显著性(P<0.05)。Her-2在各组阳性表达率分别为0%、29.5%和61.7%。Glut-1在各组阳性表达率分别为5%、93.2%和100%。它们分别在各实验组之间差异均具有显著性(P<0.05)。其中Her-2阳性表达与EC的肌层浸润,脉管侵犯和民族有关(P<0.05)。Glut-1阳性表达在EC的组织学分级、临床分期、脉管侵犯和肿瘤直径之间差异具有显著性(P<0.05)。结论 PTEN、Her-2和Glut-1三者可能在EC的发生和发展中共同发挥着重要的作用。三者联合检测对EC和癌前病变的诊断具有较高的价值。Her-2和Glut-1的阳性表达与患者的不良预后有关。
Purpose To explore the expression of PTEN,Her-2 and Glut-1 in endometrioid adenocarcinoma and their role in tumorigenesis.Methods EnVision immunohistochemistry was used to detect the protein expression of PTEN,Her-2 and Glut-1 in 40 cases of complex endometrial hyperplasia,44 cases of atypical endometrial hyperplasia and 60 cases of endometrioid adenocarcinoma.Results The negative expression rate of PTEN in complex endometrial hyperplasia,endometrial atypical hyperplasia and endometrial carcinoma were 42.5%,61.4% and 66.7%,respectively;there were not significant differences between complex endometrial hyperplasia and atypical endometrial hyperplasia（P0.05）,but that between endometrioid adenocarcinoma and complex endometrial hyperplasia had significant differences（P0.05）.The positive expression rate of Her-2 and Glut-1 in complex endometrial hyperplasia,endometrial atypical hyperplasia and endometrial carcinoma were 0%,29.5% and 61.7%,and 5%,93.2% and 100%,respectively.Her-2 and Glut-1 expression had significant differences among complex endometrial hyperplasia,atypical endometrial hyperplasia and endometrioid adenocarcinoma（P0.05）.The expression of Her-2 was correlated with muscle invasion,nationality and vessel invasion（P0.05）.The expression of Glut-1 had significant differences in histological grade,clinical stage,vessel invasion and tumor′s size（P0.05）.Conclusion PTEN,Her-2 and Glut-1 may play a significant role in the development of endometrioid adenocarcinoma.The combined use of them and the histologic features are valuable in distinguishing endometrioid adenocarcinoma from endometrial precancerous lesions.The positive expression of Her-2 and Glut-1 is correlated with the poor prognosis of endometrioid adenocarcinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2011年第8期823-826,共4页
Chinese Journal of Clinical and Experimental Pathology
