摘要
目的探讨磷脂酰肌醇3-激酶（P13K）在食管鳞状细胞癌（简称食管鳞癌）中的表达及其临床意义。方法采用免疫组化SP法检测91例食管鳞癌组织和10例正常食管黏膜组织中P13K蛋白的表达，采用逆转录聚合酶链反应（RT—PCR）检测30例食管鳞癌组织和癌旁正常组织中P13KmRNA的表达，并分析P13K表达与患者临床病理指标及预后的关系。结果P13K蛋白在食管鳞癌及正常食管黏膜中的阳性表达率分别为86．8％和10．O％，差异有统计学意义（P〈0．001）。P13K蛋白的表达强度与肿瘤分化程度、侵袭深度及临床分期有关（P〈0．05），而与患者性别、年龄及有无淋巴结转移无关（P〉0．05）。肿瘤分化越差，P13K蛋白表达越强（r=0．351，P〈0．05）；肿瘤侵袭程度越深，P13K蛋白表达越强（r=0．210，P〈0．05）；肿瘤临床分期越晚，P13K蛋白表达率越高（r=0．240，P〈0．05）。RT—PCR结果显示，P13KmRNA在食管鳞癌和癌旁正常组织中的相对表达量分别为0．675±0．029和0．349±0．073，差异有统计学意义（P〈0．05）。P13K mRNA的表达与肿瘤分化程度、侵袭深度和临床分期有关（P〈0．05）。单因素回归分析显示，P13K蛋白表达、分化程度、侵袭深度、有无淋巴结转移、临床分期、有无脉管瘤栓、残端是否阳性与预后相关（均P〈0．05）。Cox多因素回归模型分析显示，肿瘤临床分期、侵袭深度、残端是否阳性与患者预后相关（均P〈0．05），P13K蛋白表达与患者预后无关（r=1．235，P=0．266）。结论P13K蛋白和mRNA在食管鳞癌组织中的表达水平明显增高，P13K基因在食管鳞癌发生及发展中起促进作用。P13K蛋白表达增强提示食管鳞癌患者预后差，可以作为判断食管鳞癌预后的辅助指标。
Objective To explore the expression of PI3K in esophageal squamous cell carcinoma （ESCC） and its clinical significance. Methods The expression of PI3K proteins was detected by immunohistochemical SP staining in 91 ESCC tissues and normal mucosa of 10 cases. RT-PCR was used to detect the expression of PI3K mRNA in 30 ESCC tissues and adjacent normal mucosa. The relation between PI3K expression and the clinicopathological features and prognosis was analyzed. Results The positive rate of expression of PI3K protein in ESCC was 86.8%, significantly higher than that in normal esophageal mucosa （10.0% ,P 〈 0. 001 ）. There was a positive correlation between P13K expression and the degrees of differentiation, invasion depth, and clinical stage. No positive correlation was found between the expression of PI3K protein and age, gender and lymph node metastasis （P 〉 0.05）. The poorer is the differentiation of ESCC, the stronger is expression of PI3 K protein （ r = 0.351, P 〈 0.05 ） ; the deeper is the invasion depth of ESCC, the stronger is expression of PI3 K protein （r = 0.210, P 〈 0.05 ） ; and the higher is the clinical stage of ESCC, the stronger expression of PI3K protein （ r = 0.240,P 〈 0.05）. RT-PCR results demonstrated that the level of PI3K mRNA expression in ESCC was 0. 675 ± 0. 029, significantly higher than that in the adjacent normal mucosa （0. 349 ± 0. 073 ,P 〈 0.05 ）. The high expression of PI3K mRNA was correlated with poor differentiation, deep invasion and clinical stage of ESCC （ P 〈 0.05 ）. The result was consistent with SP staining immunohistochemistry. By Kaplan-Meier analyses, following factors were observed to be significantly associated with prognosis： PI3K expression, the degrees of differentiation, invasion depth, lymph node metastasis, clinical stage, tumor embolus and stump invaded （ all P 〈 0.05 ）. The multivariate analysis showed that clinical stage （ P 〈 0.05 ）, invasion depth （P 〈 0.05） and stump invaded （P 〈 0.05 ） were
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2011年第8期594-598,共5页
Chinese Journal of Oncology
基金
基金项目：河北省普通高校强势特色学科（200552）
河北省卫生厅2009年医学科学研究重点课题计划（200930,20090485）
河北省自然基金（C2009001209）
关键词
食管肿瘤
磷脂酰肌醇3-激酶
预后
Esophageal neoplasms
Phosphoinostitide 3-kinase
Prognosis
