摘要
建立了一种采用掩蔽试剂对有机磷农药残留测定时的基质效应进行补偿的方法。研究了其对仪器稳定性、耐受性的影响及对基质效应的补偿效果。结果显示,1.0 g/L的L-古洛糖酸γ-内酯对敏感农药甲胺磷、乙酰甲胺磷、氧乐果和久效磷的色谱行为改善显著,响应值分别提高了2.8、9.1、10.4和4.8倍,未对非敏感农药测定产生负面影响。连续测定100个样品,期间5个标样峰高和峰面积的相对标准偏差分别为2.4%～11.8%和4.3%～7.5%,方法的加标回收率为80%～120%,相对标准偏差为1.6%～13.5%。方法操作简便、补偿效果好,适用于气相色谱法测定有机磷农药残留时基质效应的补偿。
A method was developed for the overcoming matrix effects in gas chromatography analysis of organophosphorus pesticide residues in vegetable by using L-gulonic acid γ-lactone as a masking reagent.Effects of overcoming matrix effects while using L-gulonic acid γ-lactone were investigated.The result indicated that the addition of 1.0 g/L L-gulonic acid γ-lactone could significantly improve the chromatographic behaviors of susceptible pesticides,such as methamidophos,acephate,omethoate,moncrotophos,on gas chromatography,the peak intensities of four pesticides were 2.8,9.1,10.4 and 4.8 times higher than those in acetonitrile,respectively.There were no negative effects on the chromatographic behavior of unsusceptible pesticides（e.g.chlorpyrifos and parathion）.Five 0.25 mg/L standard solutions with the addition of masking reagent were injected at the beginning and every 25 samples in the sequence（100 GC injections）.The RSDs for peak heights and peak areas of five injections were 2.4%-11.8% and 4.3%-7.5%,respectively.The average recoveries of the selected pesticides were between 80% and 120% with RSDs of 1.6%-13.5%.The method was simple and effective,and could be used to overcome matrix effects in GC analysis of organophosphorus pesticide residues in vegetables.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第8期922-926,共5页
Journal of Instrumental Analysis
基金
农业部"948"重点项目(2010-G1)
中央级公益性科研院所基本科研业务费专项资金资助项目(2009RG006)
