摘要
目的采用超临界二氧化碳流体萃取与气相色谱-质技术谱分析白花丹参脂溶性成分。方法采用正交实验,以萃取温度、萃取时间、萃取压强及夹带剂浓度为考察因素,以萃取率为指标,确定白花丹参脂溶性成分超临界二氧化碳萃取的最佳条件,并用气质联用技术对脂溶性化学成分进行分析。结果白花丹参脂溶性成分最佳提取工艺为萃取温度45℃,萃取时间1.5 h,萃取压强25 MPa,乙醇浓度90%。用气质联用技术从中鉴定了22种化合物,其中丹参酮类成分以铁锈醇(5.54%)、丹参酮ⅡA(12.54%)、隐丹参酮(8.39%)及异隐丹参酮(11.92%)为主。结论白花丹参脂溶性提取物可作为一种富含丹参酮类化合物和不饱和脂肪酸的重要原材料,该实验结果为白花丹参的进一步开发利用提供理论依据。
Objective To isolate the liposoluble components in Salvia Bunge f. alba by supercritical fluid carbon dioxide and take a chemical analysis by gas chromatography mass spectrometry （GS-MS）. Methods L9 （34） orthogonal test was used to optimize the supercritical fluid extraction （SFE） of liposoluble components in Salvia Bunge f. alba based on extracting temperature, duration, pressure and concentration of entrainer and the chemical composition of which was analyzed by GS-MS. Results The optimal process was making the extraction for 1.5 h at 45 ~C, under the pressure of 25 MPa, and with 90% ethanol as a modifier. 22 compounds were identified by GC-MS, in which the main constituents were ferruginol （ 5.54% ） , tanshinon IIA （ 12. 54% ）, cryptotanshinone （ 8. 39% ）, and isocryptotanshinone （ 11. 92% ）. Conclusion The SFE technique was successfully applied to the extration of the liposoluble in Salvia Buage f. alba. The extraction could be a kind of important materials coating a lot of tanshinons and unsaturated fatty acids. The result offered a theory guidance to further empolder and utilize Salvia Bunge f. alba .
出处
《医药导报》
CAS
2011年第8期978-981,共4页
Herald of Medicine
基金
山东省教育厅资助项目(基金编号:J09LF30)
关键词
白花丹参
萃取
超临界
二氧化碳
气相色谱-质谱联用
Salvia Bunge f. alba
Supercritical fluid carbon dioxide
Gas chromatography mass spectrometry
