摘要
目的:优选纤维素酶辅助提取叶下珠黄酮提取工艺条件,为叶下珠的高效利用提供依据。方法:采用单因素试验研究料液比、提取温度、提取时间和酶浓度对叶下珠黄酮提取得率的影响,通过正交试验优选纤维素酶辅助提取叶下珠黄酮的工艺条件。结果:纤维素酶辅助提取叶下珠黄酮的最佳工艺条件为酶质量浓度4.25 g.L-1,提取温度40℃,料液比1∶30,提取时间1.5 h。在此条件下,黄酮的提取得率为1.51%。结论:采用纤维素酶辅助提取叶下珠黄酮,提取效果较好,该方法具有较好的应用前景。
Objective： To optimize the extraction process of flavone in Phyllanthus urinaria based on celluase,and provide the basis for the efficient use of P.urinaria.Method：The single factor test was performed to study the effects of the material-liquid ratio,extraction temperature,extraction time and the concentration of cellulase on the yield rate of flavone from P.urinaria.The process of extracting flavone based on cellulose was optimized with orthogonal test.Result：The results showed that the optimized extraction condition of flavone in P.urinaria based on cellulase was as following： the concentration of celluase was 4.25 g·L-1,the extraction temperature was 40 ℃,the material-liquid ratio was 1∶30 and the extraction time was 1.5 h.Under the optimized condition,the extraction ratio of flavone was high of 1.51%.Conclusion：In the present conditions,the extracting efficiency of flavone from P.urinaria based on celluase is better,and it has a promising future in application.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第16期26-28,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
叶下珠
黄酮
提取工艺
纤维素酶
Phyllanthus urinaria
flavone
extraction process
cellulase
