摘要
基于盐酸林可霉素对联吡啶钌(Ru(bpy)32+)在铂电极上电化学信号的增敏作用,结合毛细管电泳(CE),建立了毛细管电泳-联吡啶钌电致化学发光(CE-Ru(bpy)2+-ECL)测定盐酸林可霉素的新方法.考察检测电位、检测缓冲液浓度和pH值、分离电压、进样电压和进样时间、分离缓冲液的浓度和pH值等实验条件对盐酸林可霉素分离检测条件的影响,确定了检测盐酸林可霉素的实验条件.在优化的实验条件下,盐酸林可霉素在5×10-8～9×10-6 g/mL范围内呈良好线性,检出限为1.55×10-8 g/mL.该方法可用于盐酸林可霉素注射液中林可霉素的含量测定,测定的回收率为99.62%～100.31%.
A method of capillary eclctrophoresis（CE） with tris（2,2′-bipyridyl） ruthenium（Ⅱ） electro-chemiluminescence（ECL） detection has been developed for the determination of Lincomycin hydrochloride.The effects of detection potential,concentration and acidity of phosphate buffer,separation voltage,injection voltage of tris（2,2′-bipyridyl） and time,concentration and acidity of seperation buffer on the detection were investigated.Under optimized conditions,the calibration curve for Lincomycin hydrochloride showed a good linearity in the range of 5×10-8～9×10-6 g/mL,and the detection limits was 1.55×10-8 g/mL.The method was applied for the determination of lincomycin hydrochloride in the lincomycin hydrochloride injections with a recovery of 99.62%～100.31%.
出处
《郑州大学学报（工学版）》
CAS
北大核心
2011年第4期99-102,共4页
Journal of Zhengzhou University（Engineering Science）
