摘要
目的研究人牙周膜成纤维细胞(human periodontal ligament fibroblasts,HPDLF)对罗红霉素的跨膜转运,为通过根管局部及全身给药假说提供实验依据。方法罗红霉素溶液孵育HPDLF和MC3T3-E1细胞,超声破碎细胞后,高效液相色谱法(high performance liquid chromatography,HPLC)测定不同时间点的胞内药物含量,考马斯亮蓝法测定细胞蛋白总量。结果20μg/ml的罗红霉素孵育HPDLF及MC3T3-E1细胞1、5、10分钟时均无法检测出胞内药物的存在,40μg/ml的药物作用5分钟,同样无法检测。结论在本实验条件下,不能检测出HPDLF以及MC3T3-E1细胞对罗红霉素的跨膜转运。
Objective To investigate the biological transport of roxithromyein by human periodontal ligament fibroblasts （HPDLF） and to verify the hypothesis of delivering medicine to periodontium and the whole body through the root canal. Methods HPDLF and MC3T3-E1 cells were incubated in antibiotics solutions. The intraeellular antibiotics contents were measured by HPLC and the cell total protein was measured by Bradford protein assay. Results The intraeellular antibiotics content of HPDLF and MC3T3-E1 cells couldn＇t be detected at lmin, 5 min, 10 min, when incubated in 20 μg/ml and 40 μg/ml antibiotics solutions. Conclusion Roxithromyein could not be detected of transport by HPDLF in this investigation.
出处
《北京口腔医学》
CAS
2008年第3期138-141,共4页
Beijing Journal of Stomatology
关键词
人牙周膜成纤维细胞
罗红霉素
生物转运
高效液相色谱法
Human periodontal ligament fibroblasts
Roxithromycin
Biological transport
High performance liquid chromatography
