摘要
在单因素试验的基础上以乙醇浓度、液料比和微波功率为试验因素,以总酚得率为响应值,采用三因素三水平的响应面分析法进行试验。并通过还原力、DPPH清除能力和β胡萝卜素亚油酸三个体系来评价提取物的抗氧化能力。结果表明,山核桃外果皮中总酚提取的最佳工艺条件为:乙醇体积分数56.0%,料液比1∶50,微波功率502.4 W提取时间100 s;实际测得总酚得率为123.41 mg/g,与模型预测值基本相符。山核桃外果皮提取物有很强的抗氧化活性,清除DPPH自由基的能力与BHT相当。
Based on the single - factor experiment, the method of response surface analysis with 3 factors including ethanol concentration, solvent - material ratio and microwave power on the yield of total phenol was adopted. The antioxidant of extract was studied in three different systems （reducing power, I, 1 - diphenyl - 2 - pierylhydrazyl （DPPH） free radical scavenging activity and 13 - Carotene bleaching）. The optimal conditions of microwave - assisted extraction were as follows ： ethanol concentration 56.0%, solvent - material ratio 1 ： 50, microwave power 502.4 W and time 100 s;the actual value of total phenol was 123.41 mg/g,in close agreement with the value predicted by the mathematical model. The exocarp of Carya cathayensis extract obtained at the optimal conditions exhibited a strong scavenging DPPH ability comparable to BHT.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2011年第8期109-113,122,共6页
Journal of the Chinese Cereals and Oils Association
基金
浙江省自然科学基金(Y3090428
Y3100422)
浙江林学院科研发展基金(2290000027)
关键词
山核桃外果皮
总酚
提取
响应面
抗氧化
exocarp of Carya cathayensis, total phenol, extraction,response surface methodology,antioxidant
