摘要
采用RT-PCR技术克隆获得了黄河裸裂尻鱼(Schizopygopsis pylzovi)COⅠ、Ⅱ、Ⅲ基因的编码序列,并对此进行了初步分析。结果表明,黄河裸裂尻鱼COⅠ基因全长为1 551 bp,开放阅读框(ORF)由基因全长组成,编码516个氨基酸;COⅡ基因全长为691 bp,开放阅读框为690 bp,编码230个氨基酸;COⅢ基因全长为786 bp,其开放阅读框也是由基因全长组成,编码261个氨基酸。序列同源性分析显示,黄河裸裂尻鱼与其他9种鲤科鱼类3个亚基在基因和氨基酸序列上均有较高的同源性,虽然不同物种亚基基因间存在序列差异,但是所编码的氨基酸序列却趋于一致,揭示了鲤科鱼类细胞色素C氧化酶在线粒体呼吸链电子传递中的功能稳定性。黄河裸裂尻鱼与滇池金线鲃(Sinocyclocheilus grahami)在COⅠ和COⅡ亚基上均存在相同位置、相同种类和相同数量的功能位点,而且在基于COⅠ亚基基因和氨基酸序列、COⅡ及COⅢ亚基基因序列的系统发育树中聚在同一支上,表明两者具有较近的亲缘关系,该结论与裂腹鱼亚科鱼类起源于晚第三纪广泛分布于青藏地区的喜温性原始鲃类的观点相一致。
The complete sequences of genes encoding cytochrome C oxidase subunitsⅠ,Ⅱ and Ⅲ were cloned in Schizopygopsis pylzovi by RT-PCR method.The sequence analysis showed that the complete sequence of COⅠ was 1 551 bp,which consisted of the open reading frame（ORF） encoding 516 amino acid residues;the COⅡ gene was 691 bp and contained a 690-nucleotide ORF encoding 230 amino acid residues;and the COⅢ gene was 786 bp,which was composed of the ORF encoding 261 amino acid residues.The homologous analysis of sequences showed high similarity both in DNA and corresponding amino acid sequences of COⅠ,COⅡ and COⅢ between S.pylzovi and other 9 species from family Cyprinidae.Although there were sequence differences in subunit genes among different species,the corresponding amino acids tended to be consistent,suggesting functional stability of cytochrome C oxidase in electron transfer of mitochondrial respiration chain.S.pylzovi and Sinocyclocheilus grahami clustered together in the phylogenetic trees based on gene and amino acid sequences of COⅠ,and gene sequences of COⅡ and COⅢ,coupled with the same types,positions and numbers of functional sites in COⅠ and COⅡ,suggesting that there is a relatively close relationship between them,which is consistent with the conclusion that the subfamily Schizothoracinae is originated from one of the primitive Barbinae fishes widely distributed in warm drainages in Qinghai-Tibetan Plateau during the Tertiary Period.
出处
《动物学杂志》
CAS
CSCD
北大核心
2011年第4期16-24,共9页
Chinese Journal of Zoology
基金
国家自然科学基金项目(No.30860048)
教育部新世纪优秀人才支持计划项目(No.NCET-09-0861)
关键词
黄河裸裂尻鱼
细胞色素C亚基基因
序列分析
系统发育
Schizopygopsis pylzovi
Cytochrome C oxidase subunit gene
Sequence analysis
Phylogenetics
