摘要
采用PCR技术获得了中国鮡科褶鮡属(Pseudecheneis)5种鱼类及外群种类巨魾[Bagar-ius yarrelli(Sykes)]和红河纹胸鮡(Glyptothorax fukiensis honghensisLi)的线粒体部分基因序列.序列分析结果表明:间褶鮡(Pseudecheneis interm ediusChu)与平吻褶鮡(P.pavieiVail-lant)在Cyt b基因片段上完全无差异,形成单倍型,支持间褶鮡应为平吻褶鮡的同物异名.凭Cyt b基因片段构建了它们的NJ,MP和ML分子树,3棵分子树基本一致,均支持褶鮡属构成一单系群;怒江和伊洛瓦底江"黄斑褶鮡"不同样品分别聚在一起,但二水系的样品未能形成一单系;其他各种的不同样品均能分别聚在一起形成单系.怒江和伊洛瓦底江"黄斑褶鮡"的分类地位值得今后进一步研究.
Partial sequences of mitochondrial gene were obtained by PCR amplification from 5 species in genus Pseudecheneis recorded in China and out group species Bagarius yarrelli and Glyptothorax fukiensis honghensis. The results of sequence analysis showed that there was no difference in partial Cyt b sequences between Pseudecheneis intermedius and P. paviei that constituted a haplotype. This result supported that P. intermedius was the synonym of P. paviei. Molecular trees of N J, MP and ML were individually constructed based on the partial Cyt b sequences. The structure of the three types of molecular trees was basically consistent, indicating that the genus Pseudecheneis was a monophyletic group. The samples of P. sulcatus respectively got from Nujiang River and Irrawaddy River valleys could be clustered individually, whereas the samples got from different river valleys could not be clustered together. Samples of other species could be clustered respectively. The resuits implied that the taxonomic status of P. sulcatus between the Nujiang and the Irrawaddy basins deserved to further study in the future.
出处
《西南林学院学报》
2007年第3期45-51,共7页
Journal of Southwest Forestry College
基金
云南省自然科学基金(98C005M)资助项目
中国科学院昆明动物研究所细胞与分子进化开放研究实验室基金资助项目
云南省环境科学与工程创新人才联合培养基地资助项目
国家林业局西南地区生物多样性保育重点实验室(西南林学院)开放基金(KL200701)资助项目
关键词
褶鮡属
线粒体Cyt
B基因
分类
分子进化
鮡科
Pseudecheneis
mitochondrial cytochrome b gene
taxonomy
molecular evolution
Sisoridae
